Anti-PLP1 Polyclonal Antibody for WB, IF-P, ELISA - P60201

Core Product Specifications and Parameters

Biological Background: Myelin proteolipid protein Function and Localization

• Myelin proteolipid protein (PLP1), also known as lipophilin, is the major protein component of central nervous system myelin.
• It plays a critical role in the formation and maintenance of the multilamellar structure of myelin, ensuring proper nerve signal conduction.
• Alternative splicing of the PLP1 gene produces isoforms, including the classic PLP and the smaller DM20 variant, both essential for myelin integrity.
• PLP1 is a transmembrane lipoprotein localized specifically to the cell membrane and myelin membrane, with multiple transmembrane helices.
• Post-translational modifications such as palmitoylation and phosphorylation contribute to its stability and function within the compact myelin sheath.
• Mutations in PLP1 are linked to neurological disorders, including Pelizaeus-Merzbacher disease (PMD) and spastic paraplegia type 2 (SPG2), highlighting its clinical significance.
• It is classified as a reference proteome, with a molecular weight of approximately 30 kDa, consistent across species.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 102–148 of human PLP1, a region conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For western blotting, a predominant band around 30 kDa is expected; multiple bands may appear due to isoforms or post-translational modifications.
• In immunofluorescence staining, permeabilization is necessary because PLP1 is a transmembrane protein; validate staining patterns in known myelin-rich tissues.
• As a polyclonal antibody, lot-to-lot consistency should be verified; consider titrating the antibody for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, from validated antibodies and recombinant proteins to bulk ancillary reagents, supporting your entire diagnostic development pipeline. For myelin and neurological targets like PLP1, we provide one-stop sourcing—covering everything from concept to clinic with a focus on reliability and scalability. Let our expertise bridge your research and manufacturing needs, ensuring you get the right materials for your assays efficiently.