Anti-PLOD2/LH2 Rabbit Polyclonal Antibody for WB and ELISA - O00469

Core Product Specifications and Parameters

Biological Background: PLOD2/LH2 Function and Localization

• PLOD2 encodes procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, also known as lysyl hydroxylase 2 (LH2), an enzyme belonging to the 2-oxoglutarate-dependent dioxygenase family.
• The enzyme catalyzes the hydroxylation of lysine residues in Xaa-Lys-Gly sequences of collagen, forming hydroxylysine. These modified residues are critical for the attachment of carbohydrate units and for the stability of intermolecular collagen cross-links.
• PLOD2 localizes to the rough endoplasmic reticulum membrane and the cytoplasm, where it exerts its enzymatic function during procollagen processing.
• Two main isoforms exist due to alternative splicing; isoform 2 is selectively expressed in skin, lung, dura, and aorta, while both isoforms are broadly detected in other tissues.
• The protein is highly expressed in pancreas and muscle, consistent with the high collagen turnover in these tissues.
• As a member of the PLOD gene family, mutations in PLOD2 are associated with osteogenesis imperfecta and other connective tissue disorders, highlighting its role in collagen biosynthesis and integrity.
• The enzyme requires iron and vitamin C as cofactors for its dioxygenase activity, and it undergoes post-translational modifications including glycosylation and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 85 kDa; consider loading suitable protein ladders and verifying band migration in the relevant species’ lysates.
• The immunogen corresponds to amino acids 260–380, a region that may be conserved across human, mouse, and rat; nonetheless, cross-reactivity should be validated in the actual experimental system.
• ELISA applications can be explored using recombinant PLOD2 protein or cell lysates; titration of the antibody and appropriate secondary detection reagents is recommended.
• Ensure proper sample preparation for membrane proteins by using mild detergents to maintain the native conformation, as PLOD2 is associated with the rough ER membrane.
• Inclusion of positive control lysates from pancreas or muscle (high endogenous expression) may help optimize detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio assists diagnostic manufacturers and research teams in obtaining validated IVD raw materials, from concept to commercial scale. For studies on collagen modification and connective tissue disorders, we can source monoclonal and polyclonal antibodies, ancillary reagents, and offer procurement support for rare targets. Connect with us to simplify your supply chain for PLOD2/LH2 and related research tools.