Anti-PLOD2/LH2 Rabbit Monoclonal Antibody for WB, ELISA - O00469

Core Product Specifications and Parameters

Biological Background: PLOD2/LH2 Function and Localization

• PLOD2 encodes procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (lysyl hydroxylase 2, LH2), a member of the lysyl hydroxylase family that modifies collagen lysine residues.
• LH2 catalyzes the hydroxylation of lysine residues in -Xaa-Lys-Gly- sequences within collagen, forming hydroxylysine; these hydroxylysines serve as attachment sites for carbohydrates and are critical for the stability of intermolecular collagen cross-links.
• The enzyme is localized to the rough endoplasmic reticulum membrane and cytoplasm, where co-translational collagen modification occurs.
• Tissue expression is highest in pancreas and muscle; isoforms 1 and 2 are broadly expressed, with isoform 2 specifically detected in skin, lung, dura, and aorta.
• Multiple transcript variants encode different isoforms; post-translational modifications include glycosylation, phosphorylation, and iron/metal binding, with ascorbate (vitamin C) required as a cofactor.
• PLOD2 is associated with disease variants linked to osteogenesis imperfecta, and the protein serves as a reference proteome component in proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 260–380 of human PLOD2/LH2 (NP_000926.2); confirm that the epitope region is present in your target isoform and species.
• For western blot (WB), using a reducing loading buffer and a 6–8% SDS-PAGE gel is recommended to resolve the ~85 kDa protein; consider optimizing antibody concentration between 0.1–1 μg/mL.
• In ELISA applications, indirect or capture formats may be used; validate the antibody pair suitability for sandwich assays if developing quantitative tests.
• Cross-reactivity is reported with human and rat; additional species reactivity should be verified by sequence alignment of the immunogen region.
• Perform proper controls including collagen-producing cell lines (e.g., fibroblasts, osteoblasts) and negative controls (e.g., PLOD2 knockdown or knockout lysates).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, reference laboratories, and research institutions with seamless access to a comprehensive portfolio of IVD raw materials. For collagen biology and connective tissue disease research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material sourcing. Partner with CamelBio to accelerate your assay development from concept to clinic with a trusted one-stop supply partner.