Anti-PLOD1 Rabbit pAb for WB & ELISA - Q02809

Core Product Specifications and Parameters

Biological Background: PLOD1 Function and Localization

• PLOD1 encodes lysyl hydroxylase 1 (LH1), an ~84 kDa collagen-modifying enzyme belonging to the 2-oxoglutarate-dependent dioxygenase family, essential for collagen biosynthesis and connective tissue integrity.
• As part of a multiprotein complex with P3H3 and P3H4, PLOD1 catalyzes the hydroxylation of lysine residues in collagen alpha chains at -Xaa-Lys-Gly- sequences.
  Related references:
  PMID:10686424
  PMID:15854030
  PMID:8621606
• The resulting hydroxylysine residues serve as attachment sites for carbohydrate units and are critical for the formation of stable intermolecular collagen cross-links, ensuring the mechanical strength of tissues.
• PLOD1 is anchored to the membrane of the rough endoplasmic reticulum, where it hydroxylates lysine co-translationally on nascent procollagen chains.
• The enzyme requires iron and vitamin C as cofactors, and is subject to N-glycosylation; mutations in the PLOD1 gene cause Ehlers-Danlos syndrome type VI, a severe connective tissue disorder.
• Alternative splicing yields multiple isoforms, potentially modulating enzyme activity or localization in a tissue-specific manner.
• Target expression is prominent in collagen-producing cells such as osteoblasts, chondrocytes, and dermal fibroblasts.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 19–295) encompasses a highly conserved region across human, mouse, and rat PLOD1, consistent with the stated cross-reactivity; sequence alignment verification is recommended for other species.
• For Western blot, a band near 84 kDa is expected; endogenous PLOD1 may be detectable in lysates from fibroblast lines (e.g., MRC-5, NIH/3T3) or tissues rich in collagen (skin, bone, tendon).
• Consider optimizing antibody dilution and detection conditions for ELISA, using recombinant PLOD1 protein or collagen-derived peptides as coating antigens.
• The antibody is polyclonal and may exhibit varying sensitivity and specificity; validation in the relevant sample system is advised before quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dedicated one-stop procurement partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents to diagnostic developers, laboratories, and research institutes worldwide. For the PLOD1 target, which is instrumental in collagen biosynthesis and connective tissue research, our supply capabilities encompass monoclonal and polyclonal antibodies, paired antibody sets, and bulk custom reagents. We are committed to bridging the gap between target research and clinical application with reliable sourcing solutions.