Anti-PLOD1 Polyclonal Antibody for WB, ELISA - Q02809

Core Product Specifications and Parameters

Biological Background: PLOD1 Function and Localization

• PLOD1 encodes procollagen-lysine,2-oxoglutarate 5-dioxygenase 1, also known as lysyl hydroxylase 1 (LH1), a member of the PLOD family of collagen-modifying enzymes.
• It catalyzes the hydroxylation of lysine residues in collagen alpha chains, specifically converting lysine to hydroxylysine in -Xaa-Lys-Gly- sequences (PubMed:10686424, PubMed:15854030, PubMed:8621606). Related references: PMID:10686424, PMID:15854030, PMID:8621606
• Functions as part of a complex with P3H3 and P3H4, essential for proper collagen fibril assembly and inter‑molecular cross‑linking.
• Hydroxylysine residues serve as attachment sites for carbohydrate moieties, which are critical for the stability of collagen cross‑links.
• Localizes to the rough endoplasmic reticulum membrane, where collagen biosynthesis occurs.
• Requires iron and vitamin C as cofactors for its dioxygenase activity; the protein is glycosylated.
• Mutations in PLOD1 are associated with Ehlers-Danlos syndrome kyphoscoliotic type (formerly type VI), highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal fragment (aa 488–727) of human PLOD1; consider verifying reactivity in your specific sample type and species of interest.
• For Western blot, the predicted molecular weight is approximately 84‑85 kDa; note that glycosylation and other post‑translational modifications may shift the observed band.
• When performing ELISA, the antibody can be explored as a capture or detection tool; validate performance with recombinant PLOD1 or collagen‑rich lysates.
• Cross‑reactivity with mouse is indicated; if used for mouse samples, include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing experience for IVD raw materials. This anti‑PLOD1 antibody supports collagen biosynthesis and connective tissue disorder research, and we can supply matched pairs, bulk quantities, and ancillary reagents. For specialized needs, CamelBio also assists in sourcing rare‑target antibodies and custom production solutions.