Anti-PLLP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y342

Core Product Specifications and Parameters

Biological Background: PLLP Function and Localization

• Plasmolipin (PLLP), also known as plasma membrane proteolipid (PMLP) or TM4SF11, is a tetraspanin family protein that functions as a major component of the myelin sheath.
• PLLP plays an essential role in myelin membrane biogenesis and myelination, ensuring proper insulation of axons (PubMed:26002055). Related references: PMID:26002055
• In epithelial cells, PLLP is a critical regulator of apical endocytosis, which is required for normal epithelial development (By similarity).
• It mediates the basolateral-to-apical trafficking of ICAM-1 from the subapical compartment to the apical cell membrane in hepatic epithelial cells (PubMed:34999972). Related references: PMID:34999972
• The protein localizes to the cell membrane and is specifically enriched in myelin membranes and apical membranes; it is also detected in the Golgi apparatus.
• PLLP is a transmembrane protein with multiple transmembrane helices, and it undergoes phosphorylation, as indicated by proteomics identification.
• The calculated molecular weight of human PLLP is approximately 20 kDa (UniProt: Q9Y342).

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning the N-terminal region (aa 1-100) of human PLLP. This sequence shows high conservation with mouse and rat orthologs, consistent with the reported cross-reactivity.
• For Western blotting, a band near 20 kDa is expected, corresponding to the predicted molecular weight. Endogenous expression levels may vary among tissues and cell lines.
• In immunofluorescence/ICC, PLLP is anticipated to exhibit membrane-associated staining patterns, particularly in myelin-forming cells and polarized epithelial cells.
• For ELISA applications, the peptide design suggests potential utility for detecting PLLP in cell lysates or tissue extracts; consider using a recombinant protein or peptide as a positive control.
• It is recommended to pre-validate the antibody in the relevant sample system and include appropriate negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and biomedical institutions with integrated access to premium IVD raw materials, technical expertise, and supply chain solutions—from early-stage development to clinical application. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets like PLLP, supporting studies in myelin biology and epithelial cell transport. Partner with CamelBio to streamline your procurement and accelerate your diagnostic development.