Anti-PLK1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P53350

Core Product Specifications and Parameters

Biological Background: PLK1 Function and Localization

• Serine/threonine-protein kinase that performs several essential functions throughout M phase, including regulation of centrosome maturation, spindle assembly, removal of cohesins, inactivation of APC/C inhibitors, and regulation of mitotic exit and cytokinesis. Related references: PMID:11202906, PMID:12207013, PMID:12447691
• Phosphorylates a wide array of substrates (e.g., BORA, BUB1B, CCNB1, CDC25C, FOXM1) by recognizing pre-phosphorylated motifs through its POLO box domains, thereby amplifying mitotic regulatory signals. Related references: PMID:11202906, PMID:12207013, PMID:12447691
• Key for centrosome functions and bipolar spindle assembly; phosphorylates KIZ, NEDD1, and NINL to promote gamma-tubulin ring complex recruitment and centrosome organization. Related references: PMID:16980960, PMID:19509060, PMID:12852856
• Regulates mitotic exit and cytokinesis via phosphorylation of CEP55, ECT2, KIF20A/MKLP2, PRC1, and RACGAP1, creating docking sites on the central spindle for Rho GTP exchange factor ECT2 and cleavage furrow formation. Related references: PMID:12939256, PMID:16247472, PMID:17351640, PMID:19468300, PMID:19468302
• Drives G2/M transition by phosphorylating CDC25C, WEE1, PKMYT1, and CCNB1, leading to CDK1 activation; also enhances FOXM1 transcriptional activity. Related references: PMID:11202906, PMID:12447691, PMID:12524548, PMID:19160488
• Involved in kinetochore functions and sister chromatid cohesion; phosphorylates BUB1B, FBXO5, and STAG2 to regulate kinetochore attachment, spindle assembly checkpoint, and cohesin dissociation. Related references: PMID:15148369, PMID:15469984, PMID:17376779, PMID:17617734, PMID:18331714
• Exhibits dynamic subcellular localization: nucleus, centrosome, kinetochore, spindle, and midbody. Highly expressed in placenta and colon.
• Additionally contributes to DNA damage recovery, nuclear envelope breakdown, heat shock response, and cilium biogenesis through phosphorylation of various targets (e.g., DCTN1, DZIP1, HSF1). Related references: PMID:18615013, PMID:18662541, PMID:20679239, PMID:15661742, PMID:18794143, PMID:27979967, PMID:37440612, PMID:37674080

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 304–603, covering the C-terminal POLO box domains; this region is highly conserved, supporting cross-reactivity with mouse and rat PLK1.
• For Western blotting, expect a band at approximately 68 kDa; validate with appropriate positive and negative controls, and consider using cell lines synchronized in mitosis for optimal detection.
• In IHC-P and IF/ICC, optimize fixation/permeabilization conditions; PLK1 exhibits dynamic localization (centrosomes, kinetochores, midbody) that may require gentle permeabilization to preserve antigenicity.
• Since this is a polyclonal antibody, titration is essential for each application; start with 1:500–1:2000 for WB and adjust based on target abundance and detection method.
• For ELISA, the antibody may be used as either capture or detection reagent; pairing with a monoclonal anti-PLK1 could enhance specificity in sandwich assays.
• Verify cross-reactivity in your model system; though reactive with human, mouse, and rat, target expression levels vary across tissues and cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a trusted partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support PLK1-related research in cell cycle regulation, cancer biology, and assay development. Whether you need high-quality antibodies for mitosis studies or customized solutions for diagnostic kits, CamelBio is committed to delivering reliable materials and expert guidance.