Anti-PLGF Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P49763

Core Product Specifications and Parameters

Biological Background: PLGF Function and Localization

• PLGF (Placenta growth factor), encoded by the PGF gene, is a secreted heparin-binding growth factor that plays a key role in angiogenesis.
• Multiple isoforms arise from alternative splicing; isoform PlGF-1 lacks a heparin-binding domain, while PlGF-2 possesses a heparin-binding domain and interacts with neuropilins NRP1 and NRP2.
• PLGF stimulates proliferation and migration of endothelial cells, promoting angiogenesis by binding to the receptor FLT1/VEGFR-1.
• It also contributes to pathological angiogenesis, including tumor growth.
• The protein is secreted and localizes to the extracellular space.
• Tissue expression varies: PlGF-2 is predominantly found in early placenta, while PlGF-1 is detected in colon and mammary carcinomas.
• PLGF is a glycoprotein with disulfide bonds and undergoes proteolytic processing.
• It is classified as a developmental protein, differentiation factor, and mitogen, highlighting its roles in cell growth and development.

Experimental Guidance and Technical Tips

• The immunogen (aa 19–221) covers the majority of mature human PLGF; the polyclonal antibody is expected to detect all isoforms sharing this sequence.
• For Western blotting, use reducing conditions and a protein ladder encompassing 25 kDa. Validate with appropriate positive controls (e.g., placental tissue or transfected cells).
• In immunofluorescence-paraffin (IF-P), consider antigen retrieval and permeabilization, as the secreted target may require optimized fixation.
• For ELISA, test both direct and sandwich formats and pair with a validated matched antibody; pilot experiments with recombinant PLGF protein are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, including this anti-PLGF antibody for angiogenesis, placental biology, and tumor research. Our portfolio covers validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare targets, we provide specialized sourcing and technical consultation to help move your project from concept to clinic.