Anti-PLGF Rabbit Polyclonal Antibody for WB and ELISA - P49763

Core Product Specifications and Parameters

Biological Background: PLGF Function and Localization

• PLGF (Placenta growth factor) is a member of the vascular endothelial growth factor (VEGF) family, with multiple isoforms generated by alternative splicing, including PlGF-1 and PlGF-2.
• It functions as a growth factor that stimulates angiogenesis and endothelial cell proliferation and migration, binding to its receptor FLT1/VEGFR-1.
• Isoform PlGF-2 uniquely binds to neuropilin-1 (NRP1) and neuropilin-2 (NRP2) in a heparin-dependent manner, which may modulate its activity.
• PLGF is a secreted protein, localized extracellularly to exert its paracrine and autocrine effects.
• PLGF is implicated in tumor growth promotion, highlighting its role in pathological angiogenesis.
• Tissue specificity: PlGF-2 is predominantly expressed in early (8-week) placenta, while PlGF-1 is found in colon and mammary carcinomas; all isoforms are present in most placental tissues.
• Post-translational modifications: PLGF contains disulfide bonds and is glycosylated, which are important for its stability and function.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 19-170 of human PLGF (NP_002623.2), covering the mature growth factor domain. This region may not distinguish all isoforms if the sequence is conserved across them.
• For WB: Consider the predicted molecular weight of ~25 kDa for full-length PLGF; however, isoforms and glycosylation may result in observed bands at different sizes. Validate using positive control lysates from placental or tumor tissues.
• For ELISA: The antibody could potentially be utilized as a capture or detection reagent in sandwich ELISA; pair it with a monoclonal antibody for optimal specificity. Cross-reactivity with mouse and rat PLGF is indicated, enabling use in rodent models.
• Since the antibody is a rabbit polyclonal, it may recognize multiple epitopes, offering robust signal but potentially higher background; optimize blocking and washing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For PLGF research in angiogenesis and tumor biology, CamelBio offers validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our supply chain can also assist in sourcing rare target raw materials to support your assay development and validation needs.