Anti-PLGF Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P49763

Core Product Specifications and Parameters

Biological Background: PLGF Function and Localization

• PLGF (Placenta growth factor) is a member of the vascular endothelial growth factor (VEGF) family, encoded by the PGF gene. Alternative splicing produces multiple isoforms with distinct biological properties.
• As an angiogenic growth factor, PLGF stimulates endothelial cell proliferation and migration, binding specifically to the VEGFR-1/FLT1 receptor to promote blood vessel formation.
• The PlGF-2 isoform additionally binds neuropilin-1 (NRP1) and neuropilin-2 (NRP2) in a heparin-dependent manner, modulating receptor complex formation and signaling.
• PLGF is a secreted protein, though PlGF-2 can associate with the cell surface or extracellular matrix through heparin-binding domains, influencing its bioavailability and gradient formation.
• Tissue expression varies among isoforms: PlGF-2 is specific to early placenta, while PlGF-1 is found in colon and mammary carcinomas; all isoforms are present in most placental tissues.
• Post-translational modifications include disulfide bonds and glycosylation, which are critical for proper folding and receptor activation, as anticipated for a secreted growth factor.
• The protein plays essential roles in development, differentiation, and tumor growth, making it a key target in cancer and cardiovascular research.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide from the N-terminal region (aa 1-100) of human PLGF, likely ensuring specificity for this target. Detect bands near 25 kDa in Western blotting of human, mouse, or rat samples, considering the predicted molecular weight and cross-reactivity.
• For immunofluorescence (IF-P), consider appropriate fixation and permeabilization protocols, as PLGF is secreted; intracellular accumulation may require targeted staining strategies.
• The ELISA application suggests potential for quantifying PLGF levels in biological fluids; validate assay conditions and coating concentrations in the relevant sample matrix.
• Since the epitope lies within the first 100 amino acids, the antibody may detect multiple PLGF isoforms; confirm isoform-specific patterns in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to streamline assay development. The PLGF Rabbit mAb targets the angiogenic growth factor PLGF, widely studied in cancer and developmental biology, making it a valuable addition to diagnostic and research pipelines. Leverage CamelBio's supply network to efficiently source rare targets and scale your workflows from concept to clinic.