Anti-PLG Rabbit Polyclonal Antibody for WB and ELISA - P00747

Core Product Specifications and Parameters

Biological Background: Plasminogen Function and Localization

• Plasminogen (PLG) is the zymogen form of plasmin, a serine protease that primarily degrades fibrin, the main component of blood clots (PubMed:6094526, PubMed:6919539). Upon activation, plasmin also cleaves other clot components such as thrombospondin-1 and von Willebrand factor (PubMed:24449821, PubMed:7679575). Related references: PMID:6094526 PMID:6919539 PMID:24449821 PMID:7679575
• Plasminogen contains multiple kringle domains and is activated by cleavage into the heavy and light chains to form active plasmin. It plays a role in extracellular matrix degradation, modulating tissue remodeling and inflammation (PubMed:14699093, PubMed:28849762, PubMed:9171346). Related references: PMID:14699093 PMID:28849762 PMID:9171346
• Plasminogen exhibits antiangiogenic activity, inhibiting neovascularization, possibly through interaction with ATP synthase subunit ATP5F1A (PubMed:10077593, PubMed:7525077, PubMed:9102221). Related references: PMID:10077593 PMID:7525077 PMID:9102221
• In vitro, plasminogen can cleave complement zymogens such as C1, C4, and C5 (PubMed:6447255). Related references: PMID:6447255
• During microbial infection, plasminogen binds to Borrelia burgdorferi OspC, potentially aiding bacterial dissemination, and interacts with dengue virus particles to enhance infection (PubMed:31726374). Related references: PMID:31726374
• Plasminogen is a secreted glycoprotein synthesized predominantly in the liver and present in plasma and other extracellular fluids.
• The protein undergoes phosphorylation and disulfide bond formation. It is associated with Keywords: Blood coagulation, Fibrinolysis, Hemostasis, Thrombophilia, Tissue remodeling, Zymogen.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 20-300) of human plasminogen, covering the PAN domain and kringle structures. This region is well-conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blotting, a band at approximately 91 kDa is expected, corresponding to full-length plasminogen; however, activation fragments or glycosylated forms may appear at different sizes. We recommend optimizing sample preparation and gel percentage.
• For ELISA, use the antibody for capture or detection of recombinant or native plasminogen. Titrate the antibody to determine optimal working concentration.
• Cross-reactivity with mouse and rat has been reported; validate in the relevant sample system for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting research and diagnostic development in fibrinolysis, coagulation, and tissue remodeling. Our portfolio includes validated polyclonal antibodies like this anti-plasminogen antibody, as well as matched pairs, monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, enabling streamlined development from concept to clinic.