Anti-PLEKHB2 Polyclonal Antibody for WB, IHC-P, ELISA - Q96CS7

Core Product Specifications and Parameters

Biological Background: PLEKHB2 Function and Localization

• PLEKHB2 (Pleckstrin homology domain-containing family member B 2), also known as evectin-2, is encoded by the PLEKHB2 gene (GeneID: 55041).
• It is a member of the pleckstrin homology domain-containing protein family, characterized by a PH domain that mediates membrane targeting.
• The primary function of PLEKHB2 is in retrograde transport of recycling endosomes, facilitating the movement of cargo from endosomes back to the trans-Golgi network.
• Subcellularly, PLEKHB2 localizes to the recycling endosome membrane, where it participates in membrane trafficking processes.
• Alternative splicing generates multiple isoforms, which may exhibit distinct tissue expression or functional roles (UniProt: Q96CS7).
• Keywords associated with PLEKHB2 include 3D-structure (indicating structural characterization), endosome, membrane, and reference proteome, underscoring its role in endosomal compartments.
• The molecular weight of the canonical isoform is approximately 24.7 kDa (UniProt), consistent with the observed ~25 kDa band in experimental settings.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein corresponding to amino acids 1-222 of human PLEKHB2 (NP_001094093.1). This N-terminal region includes the PH domain, so the antibody may recognize multiple isoforms sharing this domain; verify specificity in the relevant sample system.
• For Western blot (WB) under reducing conditions, a band at ~25 kDa is expected; consider using positive control lysates (e.g., HeLa or HEK293) and compare with siRNA/shRNA knockdown samples to confirm signal specificity.
• In immunohistochemistry (IHC-P), antigen retrieval methods and antibody dilution need empirical optimization; include isotype controls to assess background staining.
• For ELISA, use the purified recombinant protein as standard; cross-reactivity with other PH domain-containing proteins should be evaluated if multiplexing.
• Store the antibody as recommended (ice bag shipping, then at -20°C to -80°C upon arrival) to maintain stability; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PLEKHB2 antibody supports research into endosomal trafficking and related disorders. Through CamelBio's supply network, customers can source validated antibody pairs, bulk ancillary reagents, and customized raw-material solutions to accelerate assay development and commercialization.