Anti-PLEK Polyclonal Antibody for WB, ELISA - P08567

Core Product Specifications and Parameters

Biological Background: PLEK Function and Localization

• Pleckstrin, also known as p47 or PLEK1, is a 40 kDa protein encoded by the PLEK gene on chromosome 2.
• It is identified as the major substrate of protein kinase C in platelets, suggesting a crucial role in platelet activation and intracellular signal transduction.
• The protein contains repeat domains that are likely involved in protein-protein and membrane interactions, though the specific domains are not detailed in the current UniProt record.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity and subcellular localization.
• Pleckstrin is a reference proteome component and is frequently identified in proteomics screens, underscoring its significance as a platelet biomarker.
• Three-dimensional structural studies have been conducted, providing insights into its domain organization and potential functional mechanisms.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fusion protein covering amino acids 1-350 of human pleckstrin, making it suitable for detecting total pleckstrin levels in Western blot and ELISA formats.
• Cross-reactivity is reported for human, mouse, and rat species; consider validating the antibody in your specific sample system to confirm reactivity and optimal working dilution.
• For Western blot, a specific band around 40 kDa is expected; platelet-rich lysates or relevant cell lines can serve as positive controls.
• In ELISA, the polyclonal nature may provide high sensitivity for capturing pleckstrin from complex biological matrices, but optimization of coating and detection conditions is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to validated IVD raw materials, including monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-pleckstrin polyclonal antibody addresses a key platelet signaling target, supporting assay development for hemostasis and thrombosis research. As a comprehensive sourcing partner, CamelBio helps you secure quality raw materials and technical insights to accelerate your projects from concept to clinic.