Anti-Pleckstrin Monoclonal Antibody for WB, ELISA - P08567

Core Product Specifications and Parameters

Biological Background: Pleckstrin Function and Localization

• Pleckstrin is the major protein kinase C (PKC) substrate in platelets, playing a central role in platelet activation and signal transduction.
• It is also referred to as platelet 47 kDa protein (p47) and is encoded by the PLEK gene in humans.
• The protein contains characteristic repeat domains that are believed to mediate protein-protein interactions.
• Post-translational modifications include phosphorylation (key for its PKC-dependent regulation) and acetylation.
• Its molecular weight is approximately 40 kDa, consistent with the designation p47.
• Pleckstrin is predominantly expressed in platelets, aligning with its function in hemostasis and thrombosis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide encompassing the N-terminal region (amino acids 1–100) of human Pleckstrin. This epitope choice supports specific detection of the target.
• For Western blot, consider using platelet-rich lysates as a positive control, given the high abundance of Pleckstrin in these cells. Validate the band size (~40 kDa) with appropriate molecular weight markers.
• In ELISA applications, use recombinant Pleckstrin protein or the immunizing peptide to establish standard curves and confirm antibody binding.
• Due to the role of Pleckstrin in PKC signaling, this antibody can be a useful tool for studying phosphorylation-dependent events in platelets, though experimental conditions will require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide a comprehensive portfolio of IVD raw materials, technical services, and consultancy—supporting projects from concept to clinic. This anti-Pleckstrin monoclonal antibody, targeting a key platelet signaling molecule, is part of our expanding catalog of validated immunological reagents. Whether you need custom antibody development, bulk ancillary reagents, or rare target sourcing, our integrated sourcing solutions help accelerate your assay development and production timelines.