Anti-PLD4 Rabbit Polyclonal Antibody for WB, ELISA - Q96BZ4

Core Product Specifications and Parameters

Biological Background: PLD4 Function and Localization

• PLD4 (Phospholipase D family member 4) is a lysosomal 5'->3' exonuclease that hydrolyzes single-stranded RNA and DNA, with higher efficiency for 5' uridine and guanosine residues. It can produce nucleoside 3'-monophosphates and 5'-hydroxy fragments, playing a key role in nucleic-acid sensing by immune cells. Related references: PMID:30111894, PMID:34620855, PMID:38537643

• Through the processing of RNA and DNA, PLD4 provides molecular signals to toll-like receptors (TLRs). It cooperates with RNase T2/RNASET2 to generate 2',3'-cGMP and cytidine-rich RNA fragments that activate TLR7, highlighting its importance in innate immunity. Related references: PMID:38697119

• Besides exonuclease activity, PLD4 can catalyze transphosphatidylation between lysophosphatidylglycerol and monoacylglycerol to produce (S,S)-bis(monoacylglycero)phosphates (BMPs), which are essential for the formation of lysosomal intralumenal vesicles and lipid degradation.

• The protein is localized to multiple intracellular compartments including the lysosome, endoplasmic reticulum membrane, Golgi apparatus, early endosomes, phagosomes, and the nucleus, reflecting its dynamic roles in immune processing.

• PLD4 is predominantly expressed in plasmacytoid dendritic cells and monocytes at the protein level, consistent with its function in innate immune sensing.

• PLD4 contains a signal-anchor, transmembrane helix, and disulfide bonds, and is predicted to be a glycosylated transmembrane protein.

• Mutations in PLD4 are associated with systemic lupus erythematosus, linking its nucleic-acid processing functions to autoimmune disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 52-200 of human PLD4, which includes the N-terminal region encompassing the first transmembrane domain and part of the exonuclease domain; this design may favor recognition of native and partially denatured protein forms.
• For WB, consider a dilution range of 1:500 to 1:2000 and validate in cell lines expressing PLD4 (e.g., human dendritic cells or monocytic lines) alongside rat tissue lysates to confirm cross-reactivity.
• For ELISA, the antibody can be explored in direct or indirect formats, with optimization of coating antigen and detection conditions for each sample matrix.
• Non-specific binding can be reduced by using appropriate blocking buffers; due to the multi-compartmental localization, subcellular fractionation may be considered to enrich the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering a streamlined supply chain for antibodies like anti-PLD4 to support innate immunity and autoimmune disease research. Building on this product’s specificity, we can provide validated antibody pairs, optimized monoclonals, and bulk ancillary reagents for your diagnostic development. Through our integrated sourcing services, we assist diagnostic manufacturers worldwide in securing rare-target raw materials, technical consultation, and reliable supply—accelerating your projects from concept to clinic.