Anti-PLCXD2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q0VAA5

Core Product Specifications and Parameters

Biological Background: PLCXD2 Function and Localization

• PLCXD2 (PI-PLC X domain-containing protein 2) is a phospholipase C enzyme that catalyzes the hydrolysis of inositol from phosphatidylinositol (PI). Experimental evidence confirms this activity. Related references: PMID:22732399
• Based on sequence analysis, PLCXD2 may also hydrolyze phosphatidylinositol-4,5 bisphosphate (PIP2) to generate the second messengers inositol-1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), thereby potentially mediating intracellular signaling cascades.
• The protein localizes primarily to the nucleus, suggesting a role in nuclear lipid metabolism or signaling.
• PLCXD2 is widely expressed across human tissues, indicating a general cellular function.
• Alternative splicing of the PLCXD2 gene produces multiple transcript variants, which may yield isoforms with distinct regulatory or functional properties.
• As a hydrolase involved in lipid degradation and metabolism, PLCXD2 is also classified as a signal transducer, linking lipid turnover to downstream cellular responses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–305) of human PLCXD2, which may allow detection of full-length protein and some splice variants. Validate isoform specificity in your model system if needed.
• For Western blot, expect a band near 35 kDa based on predicted molecular weight. Optimize loading and blocking conditions for your samples.
• In immunofluorescence or ICC, PLCXD2 is predominantly nuclear; use appropriate counterstains and controls to confirm specific staining.
• ELISA applications may benefit from using recombinant PLCXD2 or cell lysates with optimized coating buffers. Cross‑reactivity with mouse and rat orthologs has been reported, but verify in your species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets such as PLCXD2. Whether you are developing assays for lipid metabolism, signal transduction, or nuclear signaling studies, we help streamline procurement from concept to clinic.