Anti-PLCH1 Polyclonal Antibody for WB and ELISA - Q4KWH8

Core Product Specifications and Parameters

Biological Background: PLCH1 Function and Localization

• Encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1 (PLC-eta-1), a member of the phospholipase C (PLC) family that hydrolyzes phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).
• Functions as a calcium-activated phosphatidylinositol-specific phospholipase C, playing a critical role in intracellular signal transduction.
• Localizes to the cytoplasm and membrane compartments, consistent with its role in lipid signaling at cellular membranes.
• Predominantly expressed in the brain, including cerebrum, cerebellum, and spinal cord, with lower expression in lung tissue.
• During embryonic development, PLCH1 is expressed in key structures such as the notochord, developing spinal cord (ventral to dorsal gradient), dorsal root ganglia, cerebellum, and dermatomyosome, suggesting roles in neurodevelopment.
• Contains multiple domains including EF-hand motifs for calcium binding and a pleckstrin homology (PH) domain, characteristic of phospholipase C enzymes (inferred from keywords: Calcium, Metal-binding, Repeat).
• Classified as a hydrolase involved in lipid degradation and metabolism pathways (Keywords: Hydrolase, Lipid degradation, Lipid metabolism).
• Associated with disease variants linked to holoprosencephaly (Keyword: Disease variant, Holoprosencephaly), highlighting its importance in developmental disorders.

Experimental Guidance and Technical Tips

• For western blot analysis, consider using a low-percentage gel (e.g., 6-8%) and extended transfer times to resolve the ~189 kDa PLCH1 protein efficiently.
• Validate antibody specificity in relevant human and rat tissue lysates, particularly brain samples, given the protein’s expression profile.
• For ELISA, titrate the antibody concentration to optimize signal, as the immunogen region (aa 1100-1400) may influence binding affinity.
• Cross-reactivity with rat has been indicated; confirm performance in your experimental system by including appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, providing access to validated antibody pairs, optimized polyclonal antibodies like this PLCH1 antibody, and bulk ancillary reagents. Whether you require rare target antibody sourcing or full support from concept to clinic, CamelBio streamlines your development in lipid signaling and neurodevelopmental research.