Anti-PLCD4 Rabbit Polyclonal Antibody for WB, ELISA - Q9BRC7

Core Product Specifications and Parameters

Biological Background: PLCD4 Function and Localization

• PLCD4 encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4, also known as phospholipase C-delta-4 (PLC-delta-4), a member of the phospholipase C family.
• It hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two second messengers: diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG activates protein kinase C (PKC), while IP3 triggers intracellular Ca2+ release.
• Beyond its phospholipase activity, PLCD4 acts as a non-receptor guanine nucleotide exchange factor (GEF) for the G protein alpha subunit GNAI3, linking it to G protein signaling.
• PLCD4 is required for the acrosome reaction in sperm during fertilization, likely by mobilizing Ca2+ in response to zona pellucida stimulation.
• Overexpression of PLCD4 up-regulates the Erk signaling pathway and promotes cell proliferation, indicating a role in cell growth and liver regeneration.
• Subcellularly, PLCD4 localizes to the cell membrane, nucleus, cytoplasm, and endoplasmic reticulum, consistent with its signaling roles.
• It is highly expressed in skeletal muscle, kidney, and moderately in intestinal tissue, and also found in corneal epithelial cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–290 of human PLCD4 (NP_116115.1), which includes the N‑terminal pleckstrin homology (PH) domain and part of the catalytic region. This region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, the predicted molecular weight is 88 kDa; however, post‑translational modifications or splice variants may result in additional bands. Validate the antibody with appropriate positive and negative controls.
• For ELISA, consider using recombinant PLCD4 protein as a standard and optimizing the antibody concentration by titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop sourcing partner for IVD raw materials, supporting projects from concept to clinic. For PLCD4 research and assay development, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing tailored to diagnostic applications. Let CamelBio streamline your supply chain with reliable, high‑quality immunoreagents.