Anti-PLCB2 (PLC-beta-2) Rabbit Polyclonal Antibody for WB, ELISA - Q00722

Core Product Specifications and Parameters

Biological Background: PLCB2 Function and Localization

• PLCB2 is a 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2, also known as phospholipase C-beta-2 (PLC-beta-2), that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to produce the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Related references: PMID:1644792 PMID:9188725
• It is a key enzyme in phospholipid metabolism and signal transduction, linking activated G protein-coupled receptors to intracellular calcium mobilization and protein kinase C activation.
• In neutrophils, PLCB2 participates in the N-formyl peptide-activated GPCR signaling pathway, promoting RASGRP4 activation by DAG and contributing to neutrophil functional responses.
• The protein contains coiled-coil domains, requires calcium and metal ions for activity, and undergoes alternative splicing.
• PLCB2 is a phosphoprotein classified as a hydrolase involved in lipid degradation; its molecular weight is approximately 134 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–199) of human PLCB2, which may be suitable for detecting full-length and potential splice variants across species.
• For Western blot, expect a band near 134 kDa in human, mouse, and rat lysates; optimize primary antibody dilution and blocking conditions (e.g., 5 % non‑fat milk or BSA) to minimize non‑specific binding.
• For ELISA, the antibody can be used as a capture or detection reagent after appropriate validation in the relevant sample matrix.
• As a polyclonal antibody, lot‑to‑lot consistency cannot be guaranteed; validate each new batch in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one‑stop access to IVD raw materials, including validated antibody pairs and monoclonal/polyclonal antibodies for targets such as PLCB2 involved in cell signaling. For your PLCB2‑related projects, we can source bulk ancillary reagents and rare‑target raw materials, helping streamline your development from concept to clinic.