Anti-PLCB1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9NQ66

Core Product Specifications and Parameters

Biological Background: PLCB1 Function and Localization

• PLCB1 encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, also designated PLC-beta-1, PLC-154, or phospholipase C-beta-1.
• The enzyme catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), thereby mediating signal transduction downstream of G protein-coupled receptors. Related references: PMID:9188725
• PLCB1 has been reported to regulate endothelial barrier function.
• The protein localizes to the nuclear membrane and cytoplasm.
• PLCB1 is classified as a key transducer in lipid metabolism, involved in lipid degradation and signaling pathways.
• Alternative splicing yields multiple PLCB1 isoforms, contributing to functional diversity.
• Mutations in PLCB1 are associated with epilepsy, and the protein undergoes post-translational modifications including phosphorylation and palmitoylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 917–1216), which may aid in detecting both full-length and C-terminal variants.
• For WB, the predicted molecular weight is ~139 kDa; consider using an appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In IHC-P, perform antigen retrieval and validate in relevant tissue samples.
• For ELISA, consider pairing with a capture antibody if used in a sandwich format.
• Cross-reactivity with mouse and rat allows testing in orthologous models; validate species-specific reactivity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-PLCB1 polyclonal antibody supports phospholipase C signaling research and assay development. For rare-target sourcing or customized technical consulting, contact CamelBio to bridge your concept-to-clinic needs.