Anti-PLC gamma 2 (PLCG2) Polyclonal Antibody for WB, IF/ICC, ELISA - P16885

Core Product Specifications and Parameters

Biological Background: PLC gamma 2 Function and Localization

• PLCγ2 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2), encoded by the PLCG2 gene, catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), playing a central role in transmembrane signal transduction. Related references: PMID:19394299 PMID:23000145
• It functions as a crucial mediator linking receptor activation to downstream calcium mobilization and protein kinase C activation, thereby regulating diverse cellular processes including proliferation, differentiation, and immune responses. Related references: PMID:23000145
• PLCγ2 contains regulatory SH2 and SH3 domains that facilitate protein-protein interactions and recruitment to phosphorylated receptors, positioning it as a key transducer in immune and inflammatory signaling pathways.
• The protein localizes to membrane rafts, specialized microdomains enriched in cholesterol and sphingolipids, where it is optimally positioned to interact with activated receptors and substrates.
• PLCγ2 is regulated by tyrosine phosphorylation, which enhances its enzymatic activity, and is further modulated by calcium and other cofactors as indicated by its keywords: Phosphoprotein, Calcium.
• Disease-associated variants of PLCG2 have been linked to autoinflammatory and immunodeficiency disorders (FCAS3, APLAID), highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-300) of human PLCγ2, which is conserved in mouse; this may contribute to cross-reactivity. Consider verifying reactivity in other species if needed.
• For Western blot (WB), the predicted protein weight is ~148 kDa; ensure adequate transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), membrane raft localization may require optimized fixation and permeabilization protocols to preserve membrane architecture.
• For ELISA, consider using the antibody as a capture or detection reagent in sandwich formats; validate with recombinant PLCγ2 or native protein from relevant cell lysates.
• Note that the antibody is a polyclonal, which may exhibit batch-to-batch variability; include appropriate controls and validate in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to validated IVD raw materials, including this anti-PLCγ2 antibody, ideal for developing signaling pathway assays. We provide comprehensive sourcing solutions—from optimized antibody pairs and bulk ancillary reagents to rare target procurement—to accelerate your project from concept to clinic.