Anti-PLC Gamma 1 (PLCG1) Rabbit Polyclonal Antibody for WB, ELISA - P19174

Core Product Specifications and Parameters

Biological Background: PLC Gamma 1 Function and Localization

• PLCG1 encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, also known as PLC-gamma-1, PLC-148, and phospholipase C-II. It is a key enzyme in intracellular signal transduction.
• Upon activation by receptor tyrosine kinases (e.g., PDGFR, EGFR, FGFR), PLCG1 hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), which respectively activate protein kinase C and mobilize intracellular calcium.
• PLCG1 plays a critical role in actin cytoskeleton reorganization and cell migration, as demonstrated in functional studies (PubMed:17229814). Related references: PMID:17229814
• The protein localizes to cell projections such as lamellipodia and ruffles, consistent with its involvement in cell motility and membrane dynamics.
• PLCG1 contains SH2 and SH3 domains, which mediate protein-protein interactions with phosphotyrosine-containing receptors and downstream effectors, and it is regulated by phosphorylation and ubiquitination.
• Alternative splicing generates multiple PLCG1 isoforms, potentially contributing to functional diversity in different tissues and developmental stages.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight is approximately 149 kDa; consider using a low-percentage acrylamide gel and adequate transfer times to ensure efficient detection.
• Validate antibody specificity in a relevant cell line (e.g., HeLa, Jurkat) using positive and negative controls. Cross-reactivity with mouse and rat samples may require species-specific optimization.
• In ELISA applications, this polyclonal antibody can be used as a capture or detection reagent. Pairing with a well-matched monoclonal antibody is recommended for sandwich ELISA development.
• The immunogen sequence (amino acids 700-800) lies within a region of PLCG1 that is highly conserved across human, mouse, and rat, supporting cross-species utility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier supplier of IVD raw materials, offering diagnostic manufacturers, laboratories, and research institutions end-to-end support from concept to clinic. The anti-PLCG1 antibody is an essential tool for research into cell signaling pathways with implications in cancer and immune disorders, and CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare targets to accelerate your development. For seamless procurement of high-quality raw materials tailored to your diagnostic needs, partner with CamelBio.