Anti-PLC gamma 1 (PLCG1) Rabbit Polyclonal Antibody for WB and ELISA - P19174

Core Product Specifications and Parameters

Biological Background: PLC gamma 1 Function and Localization

• Encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, also known as PLC-gamma-1, PLC-148, or PLC-II, a key enzyme in phosphoinositide signaling.
• Mediates the production of second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), which regulate intracellular calcium and protein kinase C activation.
• Activated by ligand-stimulated receptor tyrosine kinases (e.g., PDGFRA, EGFR, FGFRs) and plays a central role in growth factor signaling cascades.
• Functions in actin reorganization and cell migration, contributing to lamellipodium and ruffle dynamics. Related references: PMID:17229814
• Acts as a guanine nucleotide exchange factor (GEF) for DNM1, enhancing dynamin-dependent endocytosis.
• Localizes to cell projections, specifically lamellipodia and membrane ruffles, consistent with its role in cell motility.
• Contains SH2 and SH3 domains, undergoes alternative splicing, and is regulated by phosphorylation and ubiquitination.
• Associated with disease variants and host-virus interactions; identified in multiple proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 1202–1291) of human PLCG1. For western blotting, expect a specific band near 149 kDa; validate using appropriate controls (e.g., knockdown or tissue-specific expression).
• In ELISA applications, the antibody can be used for antigen detection or capture; optimize coating and detection conditions experimentally.
• Confirmed cross-reactivity with human and mouse PLCG1; performance in other species should be validated individually.
• Consider checking for potential band shifts due to post-translational modifications (e.g., phosphorylation) or splice variants.
• For critical assays, include a negative control (e.g., PLCG1 knockout lysate) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, supporting every stage from concept to clinic. This anti-PLCG1 antibody is part of our extensive catalog for signal transduction research, complemented by validated antibody pairs, optimized monoclonal/polyclonal options, and bulk ancillary reagents. For rare or custom targets, our sourcing team can assist in acquiring specialized raw materials to accelerate your assay development.