Anti-PLC gamma 1 (PLCG1) Rabbit Monoclonal Antibody for WB, ELISA - P19174

Core Product Specifications and Parameters

Biological Background: PLCG1 Function and Localization

• PLCG1 encodes phospholipase C gamma-1, a key enzyme that hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), thereby regulating intracellular calcium and protein kinase C signaling.
• The protein is activated downstream of receptor tyrosine kinases (e.g., PDGFRA, PDGFRB, EGFR, FGFRs) and plays a critical role in growth factor-mediated signal transduction, linking cell surface receptors to downstream cascades.
• PLCG1 participates in actin cytoskeleton reorganization and cell migration, and localizes to dynamic cell structures including lamellipodia and membrane ruffles. Related references: PMID:17229814
• In addition to its lipase activity, PLCG1 functions as a guanine nucleotide exchange factor (GEF) for dynamin-1 (DNM1), facilitating GDP dissociation and enhancing DNM1-dependent endocytosis.
• The protein contains regulatory SH2 and SH3 domains that mediate interactions with phosphotyrosine-containing proteins and proline-rich motifs, contributing to its assembly into signaling complexes.
• Post-translational modifications such as phosphorylation (on tyrosine and serine residues) and acetylation modulate its activity and interactions; alternative splicing generates multiple isoforms.
• PLCG1 is ubiquitously expressed and implicated in various disease contexts, including cancers and genetic disorders, where aberrant signaling through this pathway drives pathological processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region between amino acids 710–832 of human PLCG1, which lies within the catalytic domain; this region is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, consider using whole cell lysates from commonly used human cell lines (e.g., HeLa, Jurkat, A431), where PLCG1 is endogenously expressed at moderate levels; a band of approximately 149 kDa is expected.
• When developing an ELISA assay, validate the antibody in both direct and capture formats using recombinant protein or native target; note that antibody reactivity may be influenced by protein conformation and sample preparation.
• Optimize blocking and washing conditions to minimize background, as the antibody is a rabbit monoclonal; it is recommended to perform titration experiments for each new application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for IVD manufacturers, offering one-stop access to research-grade and bulk raw materials, including monoclonal antibodies, recombinant proteins, and ancillary reagents. This PLCG1 antibody supports signal transduction research and cancer diagnostic development, and CamelBio’s supply chain can further accommodate validated antibody pairs, optimized detection systems, and rare-target sourcing to streamline your assay development. Partner with CamelBio to efficiently bridge the gap between concept and clinical application.