Anti-PLC beta 3 (PLCB3) Polyclonal Antibody for WB, IHC-P, ELISA - Q01970

Core Product Specifications and Parameters

Biological Background: PLC beta 3 (PLCB3) Function and Localization

• Encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (PLC-beta-3), a member of the phospholipase C beta family that hydrolyzes PIP2 to generate the second messengers DAG and IP3.
• Catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), crucial for intracellular calcium release and protein kinase C activation. Related references: PMID:20966218 PMID:29122926 PMID:37172014
• Functions as a key transducer of GPCR signaling, activated by G(q)/G(11) alpha subunits downstream of receptor stimulation. Related references: PMID:20966218 PMID:37991948
• In neutrophils, participates in the N-formyl peptide GPCR pathway by promoting RASGRP4 activation through DAG, thereby regulating neutrophil functional responses (By similarity).
• May contribute to keratinocyte proliferation and differentiation control via the ERK pathway, likely in complex with OSBPL2. Related references: PMID:38701954
• Localizes to the cytoplasm, membrane, and nucleus, consistent with multiple signaling roles.
• Subject to post-translational modifications including acetylation and phosphorylation, as identified in proteomics studies.
• Undergoes alternative splicing, potentially generating protein isoforms with distinct regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1010–1234) of human PLCB3; this region may recognize all known isoforms.
• For Western blot, expect a band near 139 kDa. Use low-percentage SDS-PAGE (6–8%) for better resolution of high-molecular-weight proteins, and include appropriate positive controls (e.g., HeLa, Jurkat, or brain lysates).
• For IHC-P, antigen retrieval (heat-induced, citrate buffer pH 6.0) is often required; validate staining specificity using positive and negative tissue controls.
• In ELISA, the antibody can be used for antigen capture or detection; pairing with another clone may improve sensitivity for sandwich immunoassays.
• Cross-reactivity with mouse and rat has been confirmed; consider verifying reactivity with other species if needed.

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CamelBio provides a streamlined procurement experience for IVD raw materials, offering high-quality antibodies like this anti-PLCB3 polyclonal antibody to support research and diagnostic development. We specialize in validated antibody pairs, optimized monoclonal and polyclonal reagents, and bulk ancillary materials for targets in GPCR signaling, second messenger pathways, and other critical areas. Let us be your reliable partner from concept to clinic, ensuring efficient sourcing of rare and routine raw materials alike.