Anti-PLB1 Rabbit Polyclonal Antibody for WB, ELISA - Q6P1J6

Core Product Specifications and Parameters

Biological Background: PLB1 Function and Localization

• PLB1 (Phospholipase B1) is a calcium-independent membrane-associated enzyme that possesses phospholipase B activity, capable of completely deacylating phospholipids by hydrolyzing both sn-1 and sn-2 fatty acyl chains.
• The enzyme exhibits dual phospholipase and lysophospholipase activities, with a preference for cleaving sn-2 ester bonds over sn-1 bonds in diacylphospholipids.
• PLB1 also functions as a lipase, hydrolyzing fatty acyl chains from diacylglycerols (preferring sn-2) and triacylglycerols (without positional selectivity), and may act on long-chain retinyl esters like retinyl palmitate.
• Localized to the apical cell membrane, PLB1 is suggested to contribute to the digestion of dietary phospholipids, glycerolipids, and retinoids at the brush border, facilitating lipid absorption.
• At the protein level, PLB1 is expressed in the epidermis, highlighting a potential role in skin lipid metabolism.
• The protein is classified as an alternative splicing product, glycoprotein, and transmembrane protein, with a signal peptide and repeated sequences, as indicated by UniProt keywords.
• Its molecular weight is approximately 163 kDa (calculated), consistent with the observed protein weight.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1110-1415 of human PLB1; ensure this region is not conserved in off-target proteins when designing experiments.
• For Western blot (WB), a band around 163 kDa is expected; consider using a high-molecular-weight marker and extended transfer times for large proteins.
• When using ELISA, validate antibody binding using the recombinant immunogen or native protein in relevant sample matrices.
• As the antibody cross-reacts with mouse and rat, it may be suitable for studies in these species, but always confirm specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for diagnostic manufacturers and researchers, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. With expertise in lipid metabolism and rare targets, CamelBio streamlines the procurement of high-quality raw materials like the PLB1 antibody, supporting your assay development from concept to clinic.