Anti-PLAUR Rabbit Polyclonal Antibody for WB, ELISA - Q03405

Core Product Specifications and Parameters

Biological Background: Urokinase plasminogen activator surface receptor Function and Localization

• Urokinase plasminogen activator surface receptor (PLAUR), also known as CD87, UPAR, or monocyte activation antigen Mo3, is a heavily glycosylated, GPI-anchored membrane protein.
• PLAUR acts as the primary receptor for urokinase-type plasminogen activator (uPA), concentrating and activating uPA at the cell surface to convert plasminogen into plasmin, thus driving localized extracellular matrix degradation and remodeling. Related references: PMID:1689240, PMID:15677461
• Additionally, it binds the extracellular matrix component vitronectin, mediating cell-matrix adhesion and indirectly regulating integrin signaling pathways. Related references: PMID:17548516, PMID:25168639, PMID:28849762
• Through these dual interactions, PLAUR coordinates cell migration, proliferation, and dynamic tissue remodeling in processes such as wound healing and cancer invasion. Related references: PMID:28849762
• The receptor is predominantly anchored to the cell membrane by a GPI moiety and is enriched in invadopodium membranes; a soluble secreted form can be generated by cleavage of the GPI anchor.
• Tissue-specific expression includes neurons of the rolandic cortex (confirmed at the protein level) and broader brain regions.
• Key structural features include alternative splicing, multiple intramolecular disulfide bonds, and a signal peptide, with a calculated molecular weight of approximately 37 kDa (UniProt: 36,978 Da).

Experimental Guidance and Technical Tips

• The immunogen (aa 23-305) encompasses the full extracellular ligand-binding domain, which is highly conserved across human, mouse, and rat; this explains the reported cross-reactivity, but validation in the specific sample system is recommended.
• For Western blot analysis, expect a band around 37 kDa, though extensive N-linked glycosylation may cause slight upward mobility shifts in some cell types.
• In ELISA applications, using the recombinant protein (e.g., the immunogen sequence) as a positive control can aid assay optimization; consider pairing with a matched detection antibody for sandwich ELISA development.
• As PLAUR can exist in a soluble shed form, appropriate sample preparation (e.g., serum, cell supernatant) may require concentration or protease inhibitors to avoid degradation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop platform connecting IVD manufacturers, diagnostic laboratories, and research institutes with high-quality raw materials, technical services, and expert consulting from concept to clinic. For your PLAUR/uPAR-targeted studies in tumor invasion or tissue remodeling, we supply validated polyclonal and monoclonal antibodies, optimized bulk ancillary reagents, and have the capability to source rare targets. Contact us to explore how our integrated supply chain can accelerate your next project.