Anti-PLAU Rabbit Polyclonal Antibody for WB, ELISA - P00749

Core Product Specifications and Parameters

Biological Background: PLAU Function and Localization

• Plasminogen activation: PLAU is a serine protease that cleaves plasminogen at a specific Arg-Val bond, converting it into the active serine protease plasmin.
  Related references: PMID:1969415, PMID:2521625, PMID:4270330
• Cell-surface activation: Secreted as an inactive proenzyme, PLAU binds to the urokinase plasminogen activator receptor (uPAR) on the cell surface, where it is activated and mediates localized pericellular proteolysis, including degradation of extracellular matrix components. This activity supports cell migration and tissue remodeling.
  Related references: PMID:1829461, PMID:2521625, PMID:28849762
• Fibrinolysis: PLAU promotes fibrinolysis and blood clot clearance by generating plasmin.
• Subcellular localization: The protein is secreted into the extracellular space.
• Tissue expression: PLAU is notably expressed in the prostate gland and in prostate cancers.
• Post-translational modifications: PLAU is glycosylated and phosphorylated, and its structure includes EGF-like and kringle domains, as well as multiple disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 20–180 (N-terminal region) of human PLAU; this antibody may recognize both the pro-form and, if the epitope remains intact, certain processed forms.
• In Western blot, a predominant band near 49 kDa is expected, corresponding to the full-length pro‑enzyme, with potential additional bands from proteolytic cleavage.
• This polyclonal antibody is suitable for ELISA; its high avidity can provide sensitive detection when paired with appropriate antigens or secondary reagents.
• Cross‑reactivity with mouse and rat is indicated; when working with these species, validate performance in the relevant tissue or cell lysate before large‑scale use.
• For optimal results, titrate the antibody in your specific assay system and consider running appropriate positive controls that express PLAU.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop supplier of IVD raw materials, serving diagnostic manufacturers and research laboratories worldwide. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets, covering every stage from concept to clinic. For your PLAU-related protease and fibrinolysis studies, CamelBio offers flexible procurement of this rabbit polyclonal antibody alongside complementary raw materials tailored to your immunoassay development needs.