Anti-PLA2R1 Rabbit Polyclonal Antibody for WB, ELISA - Q13018

Core Product Specifications and Parameters

Biological Background: PLA2R1 Function and Localization

• PLA2R1 (Secretory phospholipase A2 receptor), also known as PLA2R, CLEC13C, and PLA2G1R, is a transmembrane receptor belonging to the C-type lectin family (CLEC).
• It functions as a receptor for secretory phospholipase A2 (sPLA2), specifically binding sPLA2-IB/PLA2G1B, leading to MAPK cascade activation, cell proliferation, and lipid mediator production.
• The receptor plays a dual role by also acting as an endocytic receptor that internalizes and clears extracellular sPLA2, thereby protecting cells from potent enzymatic activity.
• In podocytes, PLA2R1 binding of sPLA2-IB/PLA2G1B regulates cell survival and glomerular homeostasis; it is highly expressed in kidney and podocytes. Related references: PMID:25335547
• A soluble circulating form, generated by alternative splicing, acts as a negative regulator by sequestering sPLA2 ligands and blocking their biological functions. Related references: PMID:15611272, PMID:7721806
• Subcellularly, the full-length receptor is localized at the cell membrane, while the soluble isoform is secreted; tissue expression extends to pancreas, lung macrophages, and placenta.
• Key structural features include an N-terminal signal peptide, C-type lectin domains, a single-pass transmembrane helix, disulfide bonds, and N-linked glycosylation sites.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 870–1130 within the extracellular region of human PLA2R1, potentially targeting both the membrane-bound and soluble receptor forms.
• For Western blotting, a band near 169 kDa is expected; optimize transfer conditions for high-molecular-weight proteins and use a low-percentage gel.
• The antibody is supplied as a rabbit polyclonal and thus recognizes multiple epitopes; this can enhance signal in ELISA but may require purification or blocking strategies to reduce background.
• Cross-reactivity with mouse and rat is indicated; validate the antibody’s performance in your target tissue or cell line before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supporting diagnostic manufacturers and research laboratories with premium IVD raw materials, including anti-PLA2R1 antibodies for kidney disease and phospholipase pathway studies. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials. Let CamelBio be your procurement partner from concept to clinic.