Anti-PLA2G7 Polyclonal Antibody for WB, ELISA - Q13093

Core Product Specifications and Parameters

Biological Background: PLA2G7 Function and Localization

• PLA2G7 encodes platelet-activating factor acetylhydrolase (PAF-AH), also known as Lp-PLA2 or group-VIIA phospholipase A2. It is a secreted lipoprotein-associated calcium-independent enzyme that plays a key role in phospholipid catabolism during inflammatory and oxidative stress responses (PubMed:10066756, PubMed:16371369, PubMed:17090529). Related references: PMID:10066756 PMID:16371369 PMID:17090529
• It specifically hydrolyzes short-chain fatty acyl groups at the sn-2 position of phospholipids, including the inactivation of platelet-activating factor (PAF) (PubMed:10504265, PubMed:2040620, PubMed:11590221). It also cleaves oxidized phospholipids with long fatty acyl chains carrying aldehyde groups, preventing their accumulation in LDL (PubMed:2040620, PubMed:7700381). Related references: PMID:10504265 PMID:2040620 PMID:11590221 PMID:7700381
• Within HDL particles, it hydrolyzes phospholipids bearing long-chain fatty acyl hydroperoxides, thereby protecting the vascular wall from oxylipin accumulation (PubMed:17090529). Related references: PMID:17090529
• It catalyzes the release of F2-isoprostanes from membrane phospholipids, which serve as biomarkers of cellular oxidative damage (PubMed:16371369). Related references: PMID:16371369
• Subcellular localization: Secreted into the extracellular space.
• Tissue specificity: Mainly found in plasma and secreted by macrophages (at protein level).
• Keywords: Glycoprotein, disease variant (asthma), hydrolase, lipid degradation, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 51-240 of human PLA2G7, a region likely conserved; thus, cross-reactivity with mouse is expected. For Western blot, it is recommended to optimize antibody dilution using a range (e.g., 1:500–1:2000) with appropriate positive control lysates (e.g., human plasma or macrophage cell lines).
• For ELISA, consider using recombinant human PLA2G7 protein to establish a standard curve, and validate the assay in relevant biological matrices such as serum or plasma.
• Since the antibody is polyclonal, it may detect multiple epitopes; ensure that the observed band in WB corresponds to the predicted molecular weight (~50 kDa) and consider using a blocking buffer with low cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for assay development. For targets like PLA2G7 involved in inflammation and cardiovascular disease, our team can source or produce high-quality raw materials to accelerate your diagnostic kit development from concept to clinic. We also offer rare target sourcing and technical consulting to support your entire product lifecycle.