Anti-PLA2G6 Rabbit Polyclonal Antibody for WB and ELISA - O60733

Core Product Specifications and Parameters

Biological Background: PLA2G6 Function and Localization

• PLA2G6 encodes an 85/88 kDa calcium-independent phospholipase A2 (also known as iPLA2-beta or PNPLA9) that belongs to the patatin-like phospholipase family and plays key roles in phospholipid remodeling, membrane homeostasis, mitochondrial integrity, and signal transduction.
• It functions as a phospholipase A1/A2, hydrolyzing fatty acyl chains at the sn-1 and sn-2 positions of glycerophospholipids with preference for sn-2, and also exhibits lysophospholipase activity. Related references: PMID:10092647, PMID:10336645, PMID:20886109
• Under oxidative stress, PLA2G6 remodels mitochondrial phospholipids by preferentially hydrolyzing oxidized polyunsaturated fatty acyl chains from cardiolipins to repair oxidized lipids, thereby maintaining mitochondrial integrity. Related references: PMID:23533611
• It possesses thioesterase activity toward fatty-acyl CoA, facilitating fatty acid transport and beta-oxidation in mitochondria, particularly in skeletal muscle. Related references: PMID:20886109
• PLA2G6 regulates monocyte chemotaxis by controlling F-actin polymerization at pseudopods in response to MCP1/CCL2. Related references: PMID:18208975
• Alternative splicing produces multiple isoforms with distinct tissue distribution; some isoforms may act as negative regulators due to loss of the catalytic domain.
• Subcellular localizations include the cytoplasm, cell membrane, mitochondria, and pseudopodia, consistent with its roles in membrane dynamics and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 151–250) lies within a conserved region of PLA2G6; the antibody may recognize multiple isoforms including the catalytically active forms.
• For western blotting, expect a band near the reported 90 kDa molecular weight, noting that UniProt lists an 85/88 kDa form as well due to potential post‑translational modifications or isoform variation.
• Cross‑reactivity with rat has been reported; validate the antibody in your specific sample matrix and consider species‑specific sequence alignment if working with other organisms.
• ELISA is a validated application; consider using this antibody in sandwich or indirect ELISA formats with appropriate secondary detection systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti‑PLA2G6 polyclonal antibody as part of our portfolio for researchers investigating phospholipid metabolism, mitochondrial biology, and neurodegeneration. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and can assist in sourcing rare‑target materials — from concept to clinic. For custom or volume requirements, our team is ready to support your diagnostic development pipeline.