Anti-PLA2G4D/cPLA2delta Polyclonal Antibody for IF/ICC, ELISA - Q86XP0

Core Product Specifications and Parameters

Biological Background: PLA2G4D Function and Localization

• PLA2G4D encodes cytosolic phospholipase A2 delta, a calcium-dependent enzyme that selectively hydrolyzes glycerophospholipids at the sn-2 position, with a preference for linoleic acid (PubMed:14709560). Related references: PMID:14709560
• It plays a role in lipid degradation and metabolism, potentially contributing to the generation of lipid mediators such as arachidonic acid.
• Subcellular localization includes the cytoplasm, cytosol, and membrane; membrane association is likely calcium-dependent.
• Tissue expression is restricted to stratified squamous epithelia, such as skin and cervix; it is strongly upregulated in psoriatic epidermis but absent in normal epidermis (PubMed:14709560). Related references: PMID:14709560
• The protein contains calcium-binding and metal-binding domains, essential for its enzymatic activity.
• Alternative splicing generates multiple transcript variants, potentially producing isoforms with distinct functions or localizations.
• Keywords from UniProt include hydrolase, lipid degradation, metal-binding, and reference proteome, highlighting its established role in lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 510-680), which may detect full-length and certain isoforms; validate in the relevant sample system.
• For IF/ICC, consider optimizing fixation and permeabilization to preserve antigen accessibility, especially for membrane-associated targets.
• For ELISA, use a suitable coating buffer and validate specificity with recombinant protein or cell lysates from expressing tissues.
• Since PLA2G4D is expressed in stratified epithelia, skin or cervix cell lines may serve as positive controls; normal skin may show low/absent signal.
• Avoid over-expressing the target without calcium, as localization and activity are calcium-dependent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, including validated PLA2G4D antibodies and ancillary reagents for inflammatory disease research. Our supply chain ensures timely delivery of bulk polyclonal antibodies, recombinant proteins, and customized solutions to support your diagnostic development from concept to clinic.