Anti-PLA2G4C Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q9UP65

Core Product Specifications and Parameters

Biological Background: PLA2G4C Function and Localization

• PLA2G4C encodes cytosolic phospholipase A2 gamma, a calcium-independent enzyme with phospholipase, lysophospholipase, and O-acyltransferase activities involved in phospholipid remodeling. Related references: PMID:10085124 PMID:10358058 PMID:19501189
• It catalyzes the hydrolysis of the sn-2 ester bond of phospholipids with choline and ethanolamine head groups and transfers fatty acyl groups between lysophospholipids, contributing to deacylation-reacylation cycles. Related references: PMID:15944408 PMID:19501189
• The protein localizes to multiple membrane compartments, including the cell membrane, endoplasmic reticulum, mitochondrial membranes, and lipid droplets, consistent with its role in membrane homeostasis and lipid droplet biogenesis.
• PLA2G4C is highly expressed in heart and skeletal muscle, suggesting tissue-specific functions in these energy-demanding tissues.
• Post-translational modifications include alternative splicing, methylation, phosphorylation, and prenylation, which may regulate enzyme activity and subcellular targeting.
• During hepatitis C virus (HCV) infection, PLA2G4C facilitates viral replication by remodeling host ER membranes into membranous webs and promotes virion assembly on lipid droplets. Related references: PMID:23015700 PMID:28336330
• It also enhances human T-lymphotropic virus type 1 (HTLV-1) infection by promoting leukotriene B4 biosynthesis, which acts as a chemoattractant for infected CD4+ T cells, facilitating cell-to-cell viral transmission.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–189) of human PLA2G4C; consider epitope accessibility and protein conformation when selecting assay conditions.
• For immunocytochemistry/immunofluorescence (IF/ICC), validate in appropriate PLA2G4C-expressing cell lines (e.g., human heart or skeletal muscle-derived cells) and optimize fixation/permeabilization protocols to preserve membrane localization.
• In ELISA applications, use recombinant or native PLA2G4C as a standard and titrate the antibody to determine optimal working concentration.
• As a polyclonal antibody, lot-to-lot variability may occur; include appropriate controls and validate consistency for quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with comprehensive access to IVD raw materials and technical support. For researchers studying cytosolic phospholipase A2 gamma in lipid metabolism and viral pathogenesis, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our one-stop platform simplifies sourcing of rare targets and large-volume orders, accelerating your project from concept to clinic.