Anti-PLA2G3 Rabbit Polyclonal Antibody for WB and ELISA - Q9NZ20

Core Product Specifications and Parameters

Biological Background: PLA2G3 Function and Localization

• Group 3 secretory phospholipase A2 (GIII sPLA2, sPLA2-III) is a calcium-dependent enzyme that hydrolyzes the sn-2 ester bond of extracellular phospholipids without head group selectivity.
  Related references:
  PMID:12522102
  PMID:15863501
  PMID:18801741
• Contributes to phospholipid remodeling of LDL and HDL particles, releasing unsaturated fatty acids that regulate macrophage differentiation into foam cells.
  Related references:
  PMID:18801741
• Secreted by immature mast cells, it acts on fibroblasts to promote prostaglandin D2 synthesis, which in turn enhances mast cell maturation and degranulation.
  Related references:
  PMID:23624557
• Negatively regulates ciliogenesis at the pericentrosomal preciliary compartment by modulating endocytic recycling of ciliary membrane proteins.
  Related references:
  PMID:20393563
• Coupled to the cyclooxygenase pathway, it provides arachidonate for the generation of prostaglandin E2, a potent mediator in inflammation and tumorigenesis.
  Related references:
  PMID:12522102
  PMID:15863501
• At the colonic epithelial barrier, it preferentially hydrolyzes phospholipids containing arachidonate and docosahexaenoate, contributing to the production of oxygenated metabolites that maintain colonic stem cell homeostasis.
  Related references:
  PMID:28947740
• Promotes neurite outgrowth and neuron survival by releasing specific lysophosphatidylcholine species from neuronal plasma membranes.
  Related references:
  PMID:17868035
• Subcellularly, it is secreted and also found at the cell membrane, cytoplasm, centrosome, centriole, recycling endosome, and cytoskeleton. Expressed in multiple tissues including kidney, heart, liver, and skeletal muscle, with upregulation in certain cancers and inflammatory conditions.
  Related references:
  PMID:15863501
  PMID:18801741
  PMID:23624557
  PMID:17868035

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 400–500 of human PLA2G3; this internal region may allow detection of full-length protein as well as potential cleavage products or isoforms.
• For Western blotting, expect a band near 57 kDa. Optimize protein extraction methods and include appropriate loading controls for your sample type.
• In ELISA applications, this polyclonal antibody can serve as a detection reagent; consider pairing with a matched capture antibody for sandwich assays.
• As the antibody cross-reacts with mouse, validate its performance in mouse-derived samples under your own experimental conditions to confirm specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, bridging the gap from concept to clinic. For research involving PLA2G3 and phospholipase A2–mediated pathways, this polyclonal antibody is an example of our validated offerings. We support diagnostic manufacturers with optimized antibody pairs, bulk ancillary reagents, and customized sourcing of rare-target raw materials to streamline your development workflows.