Anti-PLA2G2D Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UNK4

Core Product Specifications and Parameters

Biological Background: PLA2G2D Function and Localization

• Group IID secretory phospholipase A2 (PLA2G2D; also known as PLA2IID) is a calcium-dependent secreted enzyme that hydrolyzes phospholipids to release free fatty acids and lysophospholipids, exerting anti-inflammatory and immunosuppressive functions. Related references: PMID:10455175 PMID:10681567
• Shows substrate preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Related references: PMID:10455175
• In lymph nodes, selectively hydrolyzes diacyl and alkenyl forms of phosphatidylethanolamines, releasing omega-3 PUFAs that act as precursors for anti-inflammatory resolvins and drive resolution of inflammation.
• Promotes regulatory T cell (Treg) differentiation and immune tolerance via a catalytic-independent mechanism.
• Has bactericidal activity against Gram-positive bacteria by directly hydrolyzing bacterial membrane phospholipids.
• Secreted protein highly expressed in pancreas and spleen, with moderate levels in colon, thymus, placenta, small intestine, and prostate.
• Contains disulfide bonds and is glycosylated; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 21-145, representing the mature secreted form of PLA2G2D after signal peptide cleavage. This design may enhance detection of the endogenous secreted protein.
• For Western blot, consider using pancreas or spleen lysates as positive controls based on reported high expression of PLA2G2D.
• In IHC-P, include appropriate antigen retrieval and validate staining patterns in known expression sites such as pancreas or lymphoid tissues.
• For IF/ICC, cells that secrete PLA2G2D or immune cells undergoing activation may serve as relevant models; consider co-staining with established secretion markers.
• Cross-reactivity with rat PLA2G2D has been reported; validate in rat tissues to confirm specificity if used in rodent studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, technical services, and consulting for diagnostic manufacturers, labs, and research institutes. This anti-PLA2G2D polyclonal antibody supports research into inflammatory resolution and immune regulation. Our supply portfolio includes validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing.