Anti-PLA2G2D Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UNK4

Core Product Specifications and Parameters

Biological Background: PLA2G2D Function and Localization

• Protein identity: PLA2G2D, also known as SPLASH, sPLA2S, PLA2IID, sPLA2-IID, is a group IID secretory phospholipase A2 encoded by the PLA2G2D gene.
• Catalytic activity: Calcium-dependent phospholipase A2 that hydrolyzes the sn-2 ester bond of phospholipids with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Related references: PMID:10455175
• Anti-inflammatory function: Exerts anti-inflammatory and immunosuppressive effects by targeting extracellular lipids. Related references: PMID:10455175, PMID:10681567
• Resolvin precursor release: In draining lymph nodes, selectively hydrolyzes phosphatidylethanolamines to release omega-3 PUFAs (eicosapentaenoate and docosahexaenoate) that serve as precursors for anti-inflammatory resolvins, including resolvin D1, which suppresses dendritic cell activation and T-helper 1 responses.
• Treg differentiation: Promotes differentiation of regulatory T cells (Tregs) and maintains immune tolerance independently of its catalytic activity.
• Bactericidal activity: Directly kills Gram-positive bacteria by hydrolyzing bacterial membrane phospholipids.
• Subcellular localization: Secreted protein.
• Tissue distribution: Highly expressed in pancreas and spleen; also present in colon, thymus, placenta, small intestine, and prostate.

Experimental Guidance and Technical Tips

• Immunogen-based recognition: The immunogen corresponds to amino acids 21-145 of human PLA2G2D (NP_036532.1), covering the mature secreted protein after signal peptide cleavage. This antibody is expected to recognize the native secreted form.
• Western blot (WB): Under reducing conditions, a band around 17 kDa is anticipated; the presence of disulfide bonds may require careful sample preparation.
• Immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC): For detection of secreted PLA2G2D in tissue sections, permeabilization and antigen retrieval steps may be necessary; consider co-staining with extracellular markers.
• ELISA: Suitable for detecting PLA2G2D in cell culture supernatants or biological fluids; validate the assay conditions for optimal sensitivity.
• Cross-reactivity: Predicted reactivity with mouse and rat PLA2G2D based on sequence conservation; validate specificity in your experimental system.
• Functional studies: Given its role in anti-inflammatory lipid mediator production, this antibody can be used to monitor PLA2G2D expression in inflammation resolution models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined portal to access this anti-PLA2G2D antibody along with a comprehensive portfolio of IVD raw materials. With expertise in immunology and inflammation research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your diagnostic development from concept to clinic.