Anti-PLA2G2A Rabbit Monoclonal Antibody for WB, ELISA - P14555

Core Product Specifications and Parameters

Biological Background: PLA2G2A Function and Localization

• Protein identity: Phospholipase A2, membrane associated (sPLA2-IIA), encoded by the PLA2G2A gene, with aliases MOM1, PLA2B, PLA2L, and others.
• Calcium-dependent enzymatic activity: Hydrolyzes the sn-2 ester bond of phospholipids, with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Related references: PMID:10455175, PMID:10681567
• Host antimicrobial defense: Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing bacterial membrane phospholipids. Related references: PMID:10358193, PMID:11694541
• Inflammatory mediator: Upon sterile inflammation, targets mitochondrial phospholipids from activated platelets, releasing arachidonate for eicosanoid synthesis and mitochondrial DAMPs that activate innate immunity. Related references: PMID:25082876
• Intestinal stem cell regulator: Modulates Paneth cell differentiation and stem cell function by inhibiting Wnt signaling in intestinal stem cells; upon inflammation, promotes tissue regeneration via prostaglandin E2 synthesis.
• Additional lipid metabolism: Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, contributing to N-acyl ethanolamine biosynthesis. Related references: PMID:14998370
• Non-catalytic integrin ligand: Independently of phospholipase activity, binds integrins ITGAV:ITGB3, ITGA4:ITGB1, and ITGA5:ITGB1, inducing cell proliferation. Related references: PMID:18635536, PMID:25398877
• Subcellular localization: Secreted, cell membrane, and mitochondrial outer membrane.
• Tissue distribution: Widely expressed in heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, prostate, ovary, colon, and small intestine; absent from lymphoid organs and brain; present in platelets. Related references: PMID:10455175, PMID:10681567, PMID:25082876

Experimental Guidance and Technical Tips

• Because the immunogen is a synthetic peptide spanning amino acids 65–144, the antibody may recognize linear epitopes; consider denaturing conditions for Western blotting.
• For ELISA, ensure the coating antigen or capture format preserves the immunogenic region for optimal binding.
• Validate antibody specificity in biologically relevant samples known to express PLA2G2A, such as platelet lysates or intestinal tissue extracts.
• Given the cross-reactivity with mouse and rat, parallel testing in these species can provide valuable corroborative data.
• As a secreted protein, consider concentration or enrichment steps (e.g., TCA precipitation) when analyzing conditioned media or low-abundance biological fluids.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this PLA2G2A rabbit monoclonal antibody for inflammation and antimicrobial studies. As a one-stop partner, we deliver optimized antibody pairs, high-activity bulk reagents, and specialized sourcing for rare-target raw materials, accelerating your transition from concept to clinic.