Anti-PLA2G1B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P04054

Core Product Specifications and Parameters

Biological Background: Phospholipase A2 Function and Localization

• PLA2G1B encodes a secreted, calcium-dependent phospholipase A2 belonging to the group IB subfamily.
• It hydrolyzes the sn-2 ester bond of phospholipids, preferentially targeting phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines, in the intestinal tract. Related references: PMID:10681567, PMID:1420353, PMID:17603006
• May act in an autocrine and paracrine manner. Related references: PMID:25335547, PMID:7721806
• Upon binding to the PLA2R1 receptor, can regulate podocyte survival and glomerular homeostasis. Related references: PMID:25335547
• It may participate in the biosynthesis of N-acyl ethanolamines, lipid mediators involved in energy metabolism and inflammation.
• Displays anti-helminth activity by altering the membrane phospholipid composition of parasitic larvae.
• Selectively expressed in pancreas, lung, liver, and kidney, with lower levels in ovary and testis.
• The mature enzyme is secreted and autocatalytically cleaved from a zymogen; its structure is stabilized by multiple disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 23-148, covering the mature secreted form of PLA2G1B after signal peptide cleavage.
• For Western blotting, consider using a 12-15% SDS-PAGE gel under reducing conditions; the expected band is approximately 16 kDa.
• In immunohistochemistry (paraffin), validate the antibody in known positive tissues such as pancreas, and include appropriate controls.
• For ELISA applications, the antibody may be suitable for detecting secreted PLA2G1B in serum or tissue extracts; sample handling should minimize proteolysis.
• Cross-reactivity with human and mouse is reported; validate performance in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For research on phospholipid metabolism, pancreatitis, or host defense, our supply chain can source PLA2G1B detection reagents and related targets efficiently, supporting your assay development from discovery through clinical validation.