Anti-PKR/EIF2AK2 Rabbit Polyclonal Antibody for WB, ELISA - P19525

Core Product Specifications and Parameters

Biological Background: PKR/EIF2AK2 Function and Localization

• PKR/EIF2AK2 is an interferon-induced, double-stranded RNA-activated serine/threonine-protein kinase that plays a critical role in the innate immune response to viral infection. Related references: PMID:18835251 PMID:19189853 PMID:19507191
• Upon activation, it phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF2α), leading to inhibition of global protein synthesis as part of the integrated stress response, while promoting translation of stress-responsive mRNAs such as ATF4. Related references: PMID:19189853 PMID:21123651 PMID:22948139
• It exerts antiviral activity against a broad range of DNA and RNA viruses, including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV), and herpes simplex virus 1 (HHV-1). Related references: PMID:11836380 PMID:19189853 PMID:19840259
• Beyond its antiviral role, PKR regulates signal transduction, apoptosis, cell proliferation, and differentiation by phosphorylating substrates such as p53, DHX9, ILF3, IRS1, and viral US11. Related references: PMID:11836380 PMID:19229320 PMID:22214662
• PKR also exhibits tyrosine-protein kinase activity, phosphorylating CDK1 at Tyr-4 upon DNA damage, which facilitates its ubiquitination and proteasomal degradation. Related references: PMID:20395957
• It modulates multiple signaling pathways, including NF-κB and p38 MAP kinase cascades, influencing pro-inflammatory cytokine expression and cellular stress responses. Related references: PMID:22948139 PMID:23084476 PMID:23372823
• PKR can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2, and NLRC4 inflammasomes. Related references: PMID:22801494
• Subcellular localization includes the cytoplasm, nucleus, and perinuclear region. Highly expressed in immune tissues such as thymus, spleen, and bone marrow; elevated levels observed in certain cancers and Alzheimer disease brain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–270 amino acids of human PKR/EIF2AK2, encompassing the dsRNA-binding domains; it is predicted to recognize full-length PKR and possibly N-terminal isoforms. Confirm specificity in the relevant sample system.
• For Western blot, endogenous PKR is expressed in many cell lines, especially those of immune origin (e.g., THP-1, Jurkat). Expect a major band at ~62 kDa. Use appropriate positive (e.g., interferon-treated cells) and negative controls.
• For ELISA, this antibody may be used as a capture or detection reagent; preliminary titration and validation with recombinant PKR protein are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing one-stop IVD raw materials and technical services, supporting diagnostic manufacturers and research laboratories from concept to clinic. For studies on antiviral innate immunity, kinase signaling, or cancer research, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Inquire about custom procurement for rare targets or full project support.