Anti-PKR/EIF2AK2 Monoclonal Antibody for WB, IF/ICC, ELISA - P19525

Core Product Specifications and Parameters

Biological Background: PKR/EIF2AK2 Function and Localization

• PKR/EIF2AK2 is an interferon-induced double-stranded RNA-dependent serine/threonine-protein kinase that also exhibits tyrosine-protein kinase activity, playing a pivotal role in the innate immune response against viral infections (PubMed:18835251, PubMed:19189853, PubMed:19507191). Related references: PMID:18835251 PMID:19189853 PMID:19507191
• It phosphorylates the α subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2α) upon activation by viral dsRNA, leading to global inhibition of protein synthesis and preferential translation of stress response mRNAs such as ATF4, thereby suppressing viral replication (PubMed:19189853, PubMed:21123651, PubMed:22948139). Related references: PMID:19189853 PMID:21123651 PMID:22948139
• Exhibits antiviral activity against a broad spectrum of DNA and RNA viruses, including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV), and herpes simplex virus 1 (HHV-1) (PubMed:11836380, PubMed:19189853, PubMed:19840259). Related references: PMID:11836380 PMID:19189853 PMID:19840259
• Beyond translational control, PKR modulates diverse signaling pathways (p38 MAP kinase, NF-κB, insulin signaling) and transcription factors (JUN, STAT1, IRF1) to regulate pro-inflammatory cytokine and interferon gene expression (PubMed:22948139, PubMed:23084476, PubMed:23372823). Related references: PMID:22948139 PMID:23084476 PMID:23372823
• Subcellular localization includes cytoplasm, nucleus, and perinuclear region, with tissue-specific high expression in thymus, spleen, and bone marrow; elevated levels are observed in breast and colon carcinomas, correlating with tumor progression.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond), contributing to its regulation under stress and immune stimuli.
• Contains RNA-binding domains and kinase motifs, classifying it as an ATP-binding transferase with dual serine/threonine and tyrosine kinase activity, essential for antiviral defense and stress granule dynamics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from amino acids 450-551, a region within the kinase domain; consider this when assessing epitope specificity and potential isoform recognition.
• This antibody is validated for Western blotting (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA; optimal working dilutions should be empirically determined for each application and sample type.
• Because PKR is an interferon-inducible protein, pre-treatment of cells with type I interferon or viral infection mimics may enhance detection in certain experimental models.
• The predicted molecular weight of PKR is 62 kDa, but post-translational modifications (e.g., phosphorylation) may cause slight shifts; include appropriate positive controls (e.g., interferon-treated HeLa or A549 lysates).
• Cross-reactivity is confirmed for human samples; for other species, sequence alignment is recommended before use, as cross-reactivity is not guaranteed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying high-quality IVD raw materials to diagnostic manufacturers and life science research laboratories, offering one-stop access to validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our PKR/EIF2AK2 antibody is positioned to support research into innate immunity, viral pathogenesis, and stress response pathways, with reliable supply chains ensuring consistency for long-term projects. Whether you need custom conjugation, large-scale production, or technical consultation, CamelBio bridges the gap from concept to clinic, empowering your diagnostic and therapeutic development.