Anti-PKP2 Polyclonal Antibody for WB - Q99959

Core Product Specifications and Parameters

Biological Background: Plakophilin-2 Function and Localization

• Core desmosome scaffold: Required for desmosome assembly and cellular adhesion; recruits DSC2, DSG2, and JUP to cell-cell junctions. Related references: PMID:25208567, PMID:22781308
• Focal adhesion and barrier function: Regulates focal adhesion turnover and maintains gingival epithelial barrier integrity. Related references: PMID:23884246, PMID:34368962
• Cardiac electrophysiology and signaling: In cardiomyocytes, essential for heart morphogenesis, sodium current propagation via ANK3/SCN5A, and profibrotic TGFB1/p38 MAPK signaling.
• Mitochondrial and nuclear integrity: Anchors desmin to preserve mitochondrial function and nuclear envelope integrity, upregulates SIRT3, and protects against DNA damage. Related references: PMID:35959657
• ssDNA binding: Binds single-stranded DNA, suggesting a nuclear role. Related references: PMID:20613778
• Gap junction regulation: May regulate GJA1 localization to gap junctions in cardiac intercalated disks. Related references: PMID:18662195
• Antiviral host factor: Inhibits influenza A virus by disrupting polymerase complex interactions. Related references: PMID:28169297
• Expression pattern: Widely expressed with enrichment in heart (intercalated disks), gingival epithelium; localization to desmosomes, cytoplasm, and nucleus.

Experimental Guidance and Technical Tips

• The immunogen (aa 578-837) targets a central region of PKP2; epitope conservation supports cross-reactivity with human, mouse, and rat.
• For Western blot, expect a band around 97–100 kDa; use protease inhibitors to minimize degradation.
• Validate in PKP2-expressing tissues or cell lines (heart, keratinocytes, gingival epithelial cells).
• Polyclonal antibodies may detect splice variants; include knock-down or isotype controls for specificity.
• For ELISA, optimize coating antigen and antibody dilution; a starting dilution of 1:500 to 1:2000 is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers with validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for cardiac and epithelial disease research. Our supply chain supports rare target raw-material sourcing, including desmosome-related proteins like PKP2, from concept to clinic. From project initiation to clinical validation, CamelBio delivers integrated procurement and technical consulting for IVD development.