Anti-PKNOX1 Rabbit Polyclonal Antibody for WB, ELISA - P55347

Core Product Specifications and Parameters

Biological Background: PKNOX1 Function and Localization

• PKNOX1 (also known as PREP-1 or PBX/knotted homeobox 1) is a homeobox protein that functions as a transcription activator.
• It activates transcription in cooperation with PBX1A and HOXA1, contributing to the regulation of developmental gene expression.
• PKNOX1 localizes to the nucleus, consistent with its role as a transcription factor.
• The protein is ubiquitously expressed, although isoform 2 is absent in bone marrow tissues.
• PKNOX1 undergoes alternative splicing and phosphorylation, as indicated by proteomics identification and keyword annotations.
• It belongs to the homeobox gene family and plays a role in transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 322-436 of human PKNOX1, a region that may ensure specific recognition of the target protein.
• For Western blot, consider using whole-cell lysates from human or mouse cell lines known to express PKNOX1, and validate band specificity with appropriate controls.
• In ELISA applications, optimize the antibody dilution and coating conditions to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibodies and reagents for researchers and diagnostic manufacturers. This PKNOX1 antibody supports transcriptional regulation studies, and CamelBio can supply it alongside ancillary reagents and rare target materials to streamline your assay development.