Anti-PKN1 Polyclonal Antibody for WB, ELISA - Q16512

Core Product Specifications and Parameters

Biological Background: PKN1 Function and Localization

• PKN1 (serine/threonine-protein kinase N1) is a member of the protein kinase C (PKC) family, also known as PAK-1, PRK1, or PKN-alpha. It contains a catalytic kinase domain and regulatory regions including a coiled-coil domain and C2-like domain.
• Acts as an effector downstream of Rho GTPases and adrenergic signaling (ADRA1B), phosphorylating multiple substrates to regulate actin cytoskeleton dynamics, intermediate filament organization, and cell migration.
• In the nucleus, PKN1 acts as a transcriptional coactivator of the androgen receptor (AR); specifically phosphorylates histone H3 at Thr-11 (H3T11ph), leading to epigenetic activation and demethylation of H3K9me, which promotes AR-dependent gene expression.
• Phosphorylates MAPT/Tau on multiple sites, reducing microtubule binding and assembly, suggesting a role in neurodegenerative disorders such as Alzheimer's disease.
• Localizes to the cytoplasm, nucleus, endosomes, cell membrane, cleavage furrow, and midbody, consistent with its roles in signaling, mitosis, and membrane trafficking.
• Ubiquitously expressed with high levels in heart, brain, placenta, lung, skeletal muscle, kidney, and pancreas; overexpression observed in various tumor cell lines, especially breast cancer.
• Key post-translational modifications include acetylation and ubiquitination; phosphorylation sites are critical for activity.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated against an N-terminal fragment (aa 1-300) of human PKN1. It is suitable for Western blot (detecting a ~104 kDa band) and ELISA.
• Consider using appropriate positive and negative controls from relevant human or mouse cell lines/tissues. For WB, ensure full denaturation and reduction; validate in your specific sample system.
• Cross-reactivity with other isoforms or related kinases should be assessed via sequence alignment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PKN1 polyclonal antibody is part of our extensive kinase and signaling portfolio, supporting research in oncology, neurodegeneration, and cell migration. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.