Anti-PKM2-specific Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P14618

Core Product Specifications and Parameters

Biological Background: PKM2 Function and Localization

• PKM2 catalyzes the final rate-limiting step of glycolysis, converting phosphoenolpyruvate to pyruvate with ATP production, and its tetramer-dimer ratio controls the switch between energy generation and biosynthetic precursor supply critical for tumor cell survival.
  Related references: PMID:15996096, PMID:1854723, PMID:20847263
• PKM2 is the embryonic isoform with low constitutive pyruvate kinase activity, requiring allosteric activation by D-fructose-1,6-bisphosphate (FBP); it is replaced by the high-activity PKM1 isoform in most adult tissues.
  Related references: PMID:18337823, PMID:20847263
• In the nucleus, PKM2 functions as a protein kinase, phosphorylating STAT3 at Tyr-705 and histone H3 at Thr-11 to drive transcription of genes like VEGFA and CD274, linking metabolism to gene expression.
  Related references: PMID:18191611, PMID:21620138, PMID:22056988, PMID:22306293, PMID:22901803, PMID:24120661, PMID:33314660
• PKM2 promotes tumorigenesis through transcriptional co-activation with NF-κB and FOXM1, and also participates in caspase-independent cell death pathways.
  Related references: PMID:17308100, PMID:26787900, PMID:33314660
• PKM2 undergoes diverse post-translational modifications—acetylation, phosphorylation, methylation, hydroxylation, S-nitrosylation, and ubiquitination—that tune its metabolic and non-metabolic activities.
• It also acts as a cytosolic thyroid hormone-binding protein (CTHBP) and an Opa-interacting protein (OIP-3), reflecting multifunctional roles beyond glycolysis.

Experimental Guidance and Technical Tips

• The immunogen lies within amino acids 380–480 of human PKM2, a region that may differentiate it from the PKM1 splice variant; include PKM1 controls when assessing isoform specificity.
• For Western blot, expect a band at ~58kDa; use lysates from human, mouse, or rat cancer cell lines (e.g., HeLa, MCF-7) and include positive and negative controls.
• In immunofluorescence or ICC, anticipate both cytoplasmic and nuclear staining; optimize fixation and permeabilization protocols to preserve epitopes in both compartments.
• For ELISA applications, the synthetic peptide immunogen can serve as a positive control; consider pairing with a detection antibody recognizing a distinct epitope for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support diagnostic development. This PKM2-specific polyclonal antibody exemplifies our commitment to providing high-quality reagents for cancer metabolism and biomarker research. From antibody sourcing to technical consultation, CamelBio streamlines your path from concept to clinical application.