Anti-PKM1/2 Rabbit PolymAb® Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P14618

Core Product Specifications and Parameters

Biological Background: PKM1/2 Function and Localization

• PKM1/2, encoded by the PKM gene, is a pyruvate kinase that catalyzes the final rate-limiting step of glycolysis, transferring a phosphoryl group from phosphoenolpyruvate (PEP) to ADP to generate ATP. Related references: PMID:15996096, PMID:1854723, PMID:20847263
• The PKM gene produces two major isoforms through alternative splicing: the embryonic and tumor-associated PKM2, and the adult tissue-specific PKM1, which differ in their regulation and activity. Related references: PMID:18337823, PMID:20847263
• PKM2 activity is controlled by an allosteric switch between a highly active tetramer and a nearly inactive dimer; this shift dictates whether glucose is used for ATP production or diverted to biosynthetic pathways, a critical process for tumor cell proliferation and survival. Related references: PMID:15996096, PMID:1854723, PMID:20847263
• Beyond its cytoplasmic metabolic role, PKM2 translocates to the nucleus where it acts as a protein kinase, phosphorylating targets such as STAT3 (Tyr-705) and histone H3 (Thr-11) to modulate transcription, including promoting VEGFA expression in angiogenesis. Related references: PMID:18191611, PMID:21620138, PMID:22056988, PMID:22306293, PMID:22901803, PMID:24120661
• PKM2 is specifically expressed in proliferating cells such as embryonic stem cells and cancer cells, while PKM1 is predominantly found in normal adult tissues. Related references: PMID:18337823
• The protein localizes to both the cytoplasm and nucleus, consistent with its dual roles in metabolism and transcriptional regulation.
• The PKM2 protein undergoes extensive post-translational modifications—including acetylation, methylation, phosphorylation, and hydroxylation—that regulate its enzymatic activity, localization, and non‑metabolic functions.

Experimental Guidance and Technical Tips

• For Western blot and immunohistochemical applications, use cell or tissue lysates from human, mouse, or rat origins, as the antibody shows broad cross‑reactivity.
• Given the dual cytoplasmic and nuclear localization of PKM1/2, consider appropriate fractionation or co‑staining protocols in IF/ICC experiments to confirm subcellular distribution.
• When assessing PKM2‑specific functions, validate the specificity of detection by using PKM2‑deficient cell lines or siRNA‑mediated knockdown, as the immunogen spans a region conserved between isoforms.
• Optimize antibody dilutions for each application via a dilution series, as optimal working concentrations can vary across sample types and detection systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and researchers by providing a comprehensive portfolio of validated raw materials, including this anti‑PKM1/2 monoclonal antibody for cancer metabolism and glycolysis research. We offer one‑stop access to IVD antibody pairs, bulk ancillary reagents, and custom rare‑target antibody sourcing, supported by technical consultation from concept to clinical application.