Anti-PKM1/2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14618

Core Product Specifications and Parameters

Biological Background: PKM1/2 Function and Localization

• Catalyzes the final rate-limiting step of glycolysis by transferring a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP.
  Related references:
  PMID:15996096 | PMID:1854723 | PMID:20847263
• The ratio between the highly active tetrameric form and the nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production, a switch critical for tumor cell proliferation and survival.
  Related references:
  PMID:15996096 | PMID:1854723 | PMID:20847263
• The M2 isoform (PKM2), predominant during embryogenesis and in tumors, exhibits low basal pyruvate kinase activity and requires allosteric activation by D-fructose 1,6-bisphosphate. Beyond its metabolic role, PKM2 translocates to the nucleus upon signaling, where it acts as a protein kinase, phosphorylating STAT3 at Tyr-705 and histone H3 at Thr-11 to drive transcription of genes such as VEGFA and CD274, promoting angiogenesis and immune evasion.
  Related references:
  PMID:18337823 | PMID:20847263 | PMID:18191611 | PMID:21620138 | PMID:22056988 | PMID:22306293 | PMID:22901803 | PMID:24120661 | PMID:33314660 | PMID:26787900
• The M1 isoform (PKM1), expressed in adult tissues, displays constitutively high pyruvate kinase activity independent of allosteric activators, functionally replacing M2 after birth.
  Related references:
  PMID:18337823 | PMID:20847263
• Subcellular localization is dynamic: PKM1/2 resides predominantly in the cytoplasm but, in the case of PKM2, various stimuli such as EGF trigger nuclear translocation, where homodimerization enables its protein kinase functions.
  Related references:
  PMID:22056988 | PMID:22306293 | PMID:22901803
• Tissue specificity: PKM2 is specifically enriched in proliferating cells, including embryonic stem cells and cancer cells, while PKM1 is found in many adult tissues but is notably absent from tumor cells.
  Related references:
  PMID:18337823
• Post‑translational modifications, including acetylation, phosphorylation, hydroxylation, and ubiquitination, fine‑tune PKM1/2 activity, stability, and subcellular trafficking, highlighting its role as a regulatory hub in metabolism and signaling.

Experimental Guidance and Technical Tips

• The immunogen spans residues 11–221 of PKM1/2 (NP_001193728.1), a region conserved between PKM1 and PKM2; researchers should validate isoform specificity in their experimental system.
• For Western blot (WB), a band around 58 kDa is expected. Include appropriate positive and negative control lysates (e.g., cancer vs. normal tissue) to confirm reactivity.
• In immunohistochemistry (IHC-P), optimization of antigen retrieval and antibody dilution is recommended for formalin-fixed, paraffin-embedded sections.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization to preserve the dual cytoplasmic/nuclear localization of PKM2 under specific stimuli.
• In ELISA applications, determine optimal antibody concentration and coating conditions empirically using standard indirect or sandwich protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw-material procurement, connecting diagnostic developers with high-performance antibodies like this PKM1/2 polyclonal antibody. Whether you need validated antibody pairs, optimized recombinant proteins, or bulk ancillary reagents for glycolytic enzyme research, our supply chain ensures consistent quality. We also specialize in sourcing rare target raw materials to accelerate your assay development from concept to clinic.