Anti-PKD1L2 Rabbit Polyclonal Antibody for WB, ELISA - Q7Z442

Core Product Specifications and Parameters

Biological Background: PKD1L2 Function and Localization

• PKD1L2, also referred to as Polycystin-1-like protein 2 or PC1-like 2 protein, belongs to the polycystin family of proteins.
• It is predicted to function as a G protein-coupled receptor, potentially participating in signal transduction pathways.
• As a multi-pass membrane protein, PKD1L2 is characterized by several transmembrane domains, consistent with its localization at the membrane.
• Alternative splicing generates at least two isoforms; isoform 2 is specifically expressed in heart and kidney, while the full-length protein shows highest expression in skeletal muscle.
• The protein undergoes glycosylation and forms disulfide bonds, indicating its nature as a membrane glycoprotein.
• UniProt keywords annotate PKD1L2 with ion channel and transport functions, suggesting a role in ion transport across membranes.
• It is widely expressed across tissues, including brain, placenta, mammary gland, testis, lung, and liver, implying broad physiological significance.
• A signal peptide is present in the protein sequence, directing it to the secretory pathway and membrane insertion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–306) of human PKD1L2; this region is likely present in multiple isoforms, allowing broad detection.
• For Western blotting, the predicted molecular weight is approximately 273 kDa, but post-translational modifications such as glycosylation may affect migration.
• Cross-reactivity with mouse suggests potential utility in mouse models; however, validation in the appropriate tissue or cell lysate is recommended.
• When performing ELISA, optimize coating buffer and antibody concentration, and include the immunogen as a positive control to assess specificity.
• Consider using non-reducing conditions or mild sample preparation to preserve disulfide-dependent epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibodies like this anti-PKD1L2 polyclonal antibody alongside matched pairs, conjugates, and bulk ancillary reagents. For researchers focusing on polycystin-related signaling and membrane protein biology, CamelBio offers one-stop access to technical support and scalable raw-material supply to accelerate diagnostic development.