Anti-PKCε Rabbit Monoclonal Antibody for WB, ELISA - Q02156

Core Product Specifications and Parameters

Biological Background: PKCε Function and Localization

• Protein kinase C epsilon type (nPKC-epsilon), encoded by PRKCE, is a calcium-independent, DAG- and phospholipid-activated serine/threonine kinase localizing to cytoplasm, cytoskeletal structures, membrane, perinuclear region, and nucleus.
• Regulates cell adhesion, migration, and cytoskeletal reorganization by phosphorylating substrates including MARCKS, PTK2/FAK, vimentin, and keratin-8.
• Mediates cardiac functions: myocyte adhesion, contractility, and excitation coupling; persistent phosphorylation of troponin I (TNNI3) leads to dilated cardiomyopathy.
• Controls neuron morphological change and NGF-induced neurite outgrowth; modulates synaptic transmission via phosphorylation of GABRG2 and heat-sensitization of TRPV1.
• Transduces LPS/TLR4 immune signaling by activating TICAM2/TRAM and IRF3 to produce cytokines.
• Phosphorylates NLRP5/MATER in cumulus cells, potentially modulating AKT pathway activation. Related references: PMID:19542546
• Phosphorylates and activates LRRK1, regulating RAB protein-mediated intracellular trafficking. Related references: PMID:36040231
• Expressed at protein level in cumulus cells; belongs to the novel protein kinase C (nPKC) subfamily.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal kinase domain (amino acids 488-737), a region conserved across human, mouse, and rat, which supports the observed cross-reactivity.
• For Western blot, a band around 84 kDa is expected (UniProt calculated mass: ~83.7 kDa). Use appropriate positive controls (e.g., HeLa, MCF7, or cardiac tissue lysates) and verify with known inhibitors or siRNA if needed.
• In ELISA, consider coating with full-length PKCε or the immunogen fragment; confirm specificity by comparing to knockout samples or blocking peptides if available.
• Due to PKCε’s involvement in multiple signaling pathways, always validate antibody specificity in your particular cell system and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your PKCε-related kinase signaling research and diagnostic development by offering this validated monoclonal antibody alongside complementary reagents and sourcing solutions. As a dedicated one-stop provider for the IVD industry, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare-target raw materials. From concept to clinic, we streamline your procurement process to accelerate your assay development in areas such as cancer invasion, cardiac biology, or neurobiology.