Anti-PKC mu Rabbit Polyclonal Antibody for WB and ELISA - Q15139

Core Product Specifications and Parameters

Biological Background: PRKD1 (PKC mu) Function and Localization

• PRKD1 (PKC mu) is a serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, regulating MAPK8/JNK1 and Ras signaling pathways. Related references: PMID:10764790, PMID:12505989, PMID:12637538
• It maintains Golgi membrane integrity and controls protein trafficking by regulating transport vesicle fission at the trans-Golgi network (TGN) via activation of phosphatidylinositol 4-kinase beta (PI4KB). Related references: PMID:10764790, PMID:12505989, PMID:12637538
• Under oxidative stress, PRKD1 is phosphorylated at Tyr-463 by SRC-ABL1, promoting cell survival through activation of the IKK complex and subsequent nuclear translocation of NFKB1. Related references: PMID:12505989
• It plays a critical role in cell migration by regulating integrin alpha-5/beta-3 recycling and recruitment to focal adhesions. Related references: PMID:10764790, PMID:12505989, PMID:12637538
• PRKD1 mediates VEGFA-induced angiogenesis by phosphorylating HDAC5 and HDAC7, leading to their nuclear export and activation of pro-angiogenic gene expression, endothelial cell migration, and tube formation. Related references: PMID:19211839
• In cardiac hypertrophy, downstream of novel PKC signaling, PRKD1 phosphorylates HDAC5, triggering its CRM1-dependent nuclear export, MEF2A activation, and induction of hypertrophy-related genes. Related references: PMID:18332134
• In epithelial cells, it is required for flagellin-stimulated inflammatory responses by binding and phosphorylating TLR5, leading to MAPK14/p38 activation and production of inflammatory cytokines. Related references: PMID:17442957
• Localizes to the cytoplasm, cell membrane, and Golgi apparatus, particularly the trans-Golgi network.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 800–900 of human PKC mu, a region near the C-terminus that may confer specific recognition of the target protein.
• For Western blot, a band at approximately 102 kDa is expected. Validate in relevant human and mouse cell lysates, as the antibody cross-reacts with both species.
• In ELISA, consider optimizing the antigen coating and antibody dilution using recombinant protein or peptide to assess binding affinity and specificity.
• Due to the polyclonal nature, potential cross-reactivity with other PKD family members (PKD2, PKD3) should be tested if panels of cell lines with known expression are available.
• Ensure appropriate controls are included, such as lysates from cells with known PRKD1 expression or siRNA knockdown, to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD manufacturers and research laboratories, offering a comprehensive portfolio of raw materials including validated primary antibodies, recombinant proteins, and ancillary reagents. This anti-PKC mu polyclonal antibody targets a key kinase involved in cell signaling and cancer pathways, making it suitable for research applications in oncology, cardiovascular biology, and inflammation. Through our global supply network, we help customers secure bulk quantities of high-quality antibodies and custom reagents, reducing development timelines from concept to clinic.