Anti-PKC mu (PRKD1) Rabbit Monoclonal Antibody for WB and ELISA - Q15139

Core Product Specifications and Parameters

Biological Background: PKC mu Function and Localization

• Protein identity and aliases: PKC mu, officially designated serine/threonine-protein kinase D1 (PRKD1), is also known as protein kinase D (PKD), nPKC-mu, and PKCM. It belongs to the protein kinase D family and contains cysteine-rich zinc-finger domains typical of the PKC superfamily.
• DAG-mediated signaling and kinase activity: PKC mu converts transient diacylglycerol (DAG) signals into sustained physiological responses by phosphorylating EGFR, RIN1, and other substrates, thereby regulating MAPK8/JNK1, Ras, and MAPK1/3 (ERK1/2) signaling cascades. These pathways control cell proliferation, differentiation, and survival. Related references: PMID:10764790 PMID:12505989 PMID:12637538 PMID:10523301
• Golgi membrane integrity and trafficking: PKC mu acts downstream of heterotrimeric G proteins to maintain Golgi structural integrity and regulates fission of transport vesicles at the trans-Golgi network (TGN), ensuring proper secretory protein trafficking to the plasma membrane.
• Cell survival and apoptosis regulation: Under oxidative stress, phosphorylation at Tyr-463 by SRC-ABL1 promotes cell survival via IKK/NF-kappa-B activation. Conversely, caspase-3 cleavage at Asp-378 during genotoxic stress enhances its kinase activity and sensitizes cells to apoptosis. Related references: PMID:12505989 PMID:10764790
• Cell migration and osteoblast differentiation: PKC mu controls cell migration by regulating integrin recycling and focal adhesion turnover. In osteoblasts, it mediates BMP2-induced nuclear export of HDAC7, relieving repression of RUNX2 to promote differentiation. Related references: PMID:18509061
• Cardiac hypertrophy and angiogenesis: In cardiomyocytes, PKC mu phosphorylates HDAC5, triggering its nuclear export and MEF2-dependent gene expression that drives pathological hypertrophy. During VEGF-induced angiogenesis, it similarly regulates HDAC7 nuclear export to promote endothelial cell migration and tube formation. Related references: PMID:18332134 PMID:19211839
• Innate immunity and inflammatory responses: PKC mu directly binds and phosphorylates Toll-like receptor 5 (TLR5) upon flagellin stimulation, leading to MAPK14/p38 activation and inflammatory cytokine production. It also phosphorylates NLRP3 to facilitate inflammasome assembly. Related references: PMID:17442957

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 200–300 of human PKC mu (Q15139). This region is conserved in mouse and rat (as indicated by cross-reactivity), but sequence verification in your target species is advised for optimal assay design.
• For Western blotting, expect a band at approximately 102 kDa. High-molecular-weight proteins may require longer transfer times or optimized blotting conditions; loading sufficient protein and using fresh samples can reduce degradation artifacts.
• In ELISA applications, the antibody’s performance can be influenced by antigen coating concentration and detection system. Titration experiments and inclusion of appropriate controls are recommended.
• As a rabbit monoclonal antibody, it offers batch-to-batch reproducibility and high affinity, making it suitable for quantitative assays when carefully validated.
• Cross-reactivity with mouse and rat has been reported; however, the extent of reactivity may vary between applications. Confirm specificity in the relevant tissue or cell context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated B2B partner for sourcing high-quality IVD raw materials, from monoclonal and polyclonal antibodies to recombinant antigens and ancillary reagents. This anti-PKC mu monoclonal antibody is part of our extensive portfolio supporting kinase signaling, cancer biology, and cardiovascular research. Contact our team for tailored procurement solutions, bulk orders, and technical consultation to accelerate your diagnostic development.